Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin

Deepa Khare, Hyunju Choi, Sung Un Huh, Barbara Bassin, Jeongsik Kim, Enrico Martinoia, Kee Hoon Sohn, Kyung Hee Paek, Youngsook Lee, Maarten J. Chrispeels

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Plant pathogens cause huge yield losses. Plant defense often depends on toxic secondary metabolites that inhibit pathogen growth. Because most secondary metabolites are also toxic to the plant, specific transporters are needed to deliver them to the pathogens. To identify the transporters that function in plant defense, we screened Arabidopsis thaliana mutants of full-size ABCG transporters for hypersensitivity to sclareol, an antifungal compound. We found that atabcg34 mutants were hypersensitive to sclareol and to the necrotrophic fungi Alternaria brassicicola and Botrytis cinerea. AtABCG34 expression was induced by A. brassicicola inoculation as well as by methyl-jasmonate, a defense-related phytohormone, and AtABCG34 was polarly localized at the external face of the plasma membrane of epidermal cells of leaves and roots. atabcg34 mutants secreted less camalexin, a major phytoalexin in A. thaliana, whereas plants overexpressing AtABCG34 secreted more camalexin to the leaf surface and were more resistant to the pathogen. When treated with exogenous camalexin, atabcg34 mutants exhibited hypersensitivity, whereas BY2 cells expressing AtABCG34 exhibited improved resistance. Analyses of natural Arabidopsis accessions revealed that AtABCG34 contributes to the disease resistance in naturally occurring genetic variants, albeit to a small extent. Together, our data suggest that AtABCG34 mediates camalexin secretion to the leaf surface and thereby prevents A. brassicicola infection.

Original languageEnglish
Pages (from-to)E5712-E5720
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number28
DOIs
Publication statusPublished - 2017 Jul 11

Fingerprint

Arabidopsis
Hypersensitivity
Toxic Plants
Botrytis
Alternaria
Disease Resistance
Plant Growth Regulators
Poisons
Fungi
Cell Membrane
camalexin
Growth
Infection
sclareol

Keywords

  • A. brassicicola
  • ABC transporters
  • ATABCG34
  • B. cinerea
  • Camalexin

ASJC Scopus subject areas

  • General

Cite this

Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin. / Khare, Deepa; Choi, Hyunju; Huh, Sung Un; Bassin, Barbara; Kim, Jeongsik; Martinoia, Enrico; Sohn, Kee Hoon; Paek, Kyung Hee; Lee, Youngsook; Chrispeels, Maarten J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 28, 11.07.2017, p. E5712-E5720.

Research output: Contribution to journalArticle

Khare, Deepa ; Choi, Hyunju ; Huh, Sung Un ; Bassin, Barbara ; Kim, Jeongsik ; Martinoia, Enrico ; Sohn, Kee Hoon ; Paek, Kyung Hee ; Lee, Youngsook ; Chrispeels, Maarten J. / Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 28. pp. E5712-E5720.
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