Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells

Anuphon Laohavisit, Zhonglin Shang, Lourdes Rubio, Tracey A. Cuin, Anne Aliénor Véry, Aihua Wang, Jennifer C. Mortimer, Neil Macpherson, Katy M. Coxon, Nicholas H. Battey, Colin Brownlee, Ohkmae Kim, Hervé Sentenac, Sergey Shabala, Alex A R Webb, Julia M. Daviesa

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Plant cell growth and stress signaling require Ca2+ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen species that is readily converted to extracellular hydrogen peroxide and hydroxyl radicals, OH. In root cells, extracellular OH activates a plasma membrane Ca2+-permeable conductance that permits Ca2+ influx. In Arabidopsis thaliana, distribution of this conductance resembles that of annexin1 (ANN1). Annexins are membrane binding proteins that can form Ca2+-permeable conductances in vitro. Here, the Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal OH-activated Ca2+- and K+-permeable conductance. This manifests in both impaired root cell growth and ability to elevate root cell cytosolic free Ca2+ in response to OH. An OH-activated Ca2+ conductance is reconstituted by recombinant ANN1 in planar lipid bilayers. ANN1 therefore presents as a novel Ca2+-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca2+ in plants.

Original languageEnglish
Pages (from-to)1522-1533
Number of pages12
JournalPlant Cell
Volume24
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Arabidopsis
plasma membrane
Cell Membrane
calcium
Reactive Oxygen Species
Growth
cells
Annexins
Membrane Transport Proteins
NADPH Oxidase
reactive oxygen species
cell growth
Salinity
Plant Cells
Lipid Bilayers
Superoxides
Hydroxyl Radical
Hydrogen Peroxide
Blood Proteins
membrane proteins

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Laohavisit, A., Shang, Z., Rubio, L., Cuin, T. A., Véry, A. A., Wang, A., ... Daviesa, J. M. (2012). Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells. Plant Cell, 24(4), 1522-1533. https://doi.org/10.1105/tpc.112.097881

Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells. / Laohavisit, Anuphon; Shang, Zhonglin; Rubio, Lourdes; Cuin, Tracey A.; Véry, Anne Aliénor; Wang, Aihua; Mortimer, Jennifer C.; Macpherson, Neil; Coxon, Katy M.; Battey, Nicholas H.; Brownlee, Colin; Kim, Ohkmae; Sentenac, Hervé; Shabala, Sergey; Webb, Alex A R; Daviesa, Julia M.

In: Plant Cell, Vol. 24, No. 4, 01.04.2012, p. 1522-1533.

Research output: Contribution to journalArticle

Laohavisit, A, Shang, Z, Rubio, L, Cuin, TA, Véry, AA, Wang, A, Mortimer, JC, Macpherson, N, Coxon, KM, Battey, NH, Brownlee, C, Kim, O, Sentenac, H, Shabala, S, Webb, AAR & Daviesa, JM 2012, 'Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells', Plant Cell, vol. 24, no. 4, pp. 1522-1533. https://doi.org/10.1105/tpc.112.097881
Laohavisit, Anuphon ; Shang, Zhonglin ; Rubio, Lourdes ; Cuin, Tracey A. ; Véry, Anne Aliénor ; Wang, Aihua ; Mortimer, Jennifer C. ; Macpherson, Neil ; Coxon, Katy M. ; Battey, Nicholas H. ; Brownlee, Colin ; Kim, Ohkmae ; Sentenac, Hervé ; Shabala, Sergey ; Webb, Alex A R ; Daviesa, Julia M. / Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells. In: Plant Cell. 2012 ; Vol. 24, No. 4. pp. 1522-1533.
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