Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells

Anuphon Laohavisit, Zhonglin Shang, Lourdes Rubio, Tracey A. Cuin, Anne Aliénor Véry, Aihua Wang, Jennifer C. Mortimer, Neil Macpherson, Katy M. Coxon, Nicholas H. Battey, Colin Brownlee, Ohkmae K. Park, Hervé Sentenac, Sergey Shabala, Alex A.R. Webb, Julia M. Daviesa

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Plant cell growth and stress signaling require Ca2+ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen species that is readily converted to extracellular hydrogen peroxide and hydroxyl radicals, OH. In root cells, extracellular OH activates a plasma membrane Ca2+-permeable conductance that permits Ca2+ influx. In Arabidopsis thaliana, distribution of this conductance resembles that of annexin1 (ANN1). Annexins are membrane binding proteins that can form Ca2+-permeable conductances in vitro. Here, the Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal OH-activated Ca2+- and K+-permeable conductance. This manifests in both impaired root cell growth and ability to elevate root cell cytosolic free Ca2+ in response to OH. An OH-activated Ca2+ conductance is reconstituted by recombinant ANN1 in planar lipid bilayers. ANN1 therefore presents as a novel Ca2+-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca2+ in plants.

Original languageEnglish
Pages (from-to)1522-1533
Number of pages12
JournalPlant Cell
Volume24
Issue number4
DOIs
Publication statusPublished - 2012 Apr

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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    Laohavisit, A., Shang, Z., Rubio, L., Cuin, T. A., Véry, A. A., Wang, A., Mortimer, J. C., Macpherson, N., Coxon, K. M., Battey, N. H., Brownlee, C., Park, O. K., Sentenac, H., Shabala, S., Webb, A. A. R., & Daviesa, J. M. (2012). Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+-and K+-permeable conductance in root cells. Plant Cell, 24(4), 1522-1533. https://doi.org/10.1105/tpc.112.097881