Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation

Jennifer M. Gagne, Jan Smalle, Derek J. Gingerich, Joseph M. Walker, Sang-Dong Yoo, Shuichi Yanagisawa, Richard D. Vierstra

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Ubiquitination of various intracellular proteins by ubiquitin-protein ligases (or E3s) plays an essential role in eukaryotic cell regulation primarily through its ability to selectively target proteins for degradation by the 26S proteasome. Skp1, Cullin, F-box (SCF) complexes are one influential E3 class that use F-box proteins to deliver targets to a core ligase activity provided by the Skp1, Cullin, and Rbx1 subunits. Almost 700 F-box proteins can be found in Arabidopsis, indicating that SCF E3s likely play a pervasive role in plant physiology and development. Here, we describe the reverse genetic analysis of two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action. Mutations in either gene cause hypersensitivity to exogenous ethylene and its precursor 1-aminocyclopropane-1-carboxylic acid. EBF1 and -2 interact directly with ethylene insensitive 3 (EIN3), a transcriptional regulator important for ethylene signaling. Levels of EIN3 are increased in mutants affecting either EBF1 or -2, suggesting that the corresponding SCF complexes work together in EIN3 breakdown. Surprisingly, double ebf1 ebf2 mutants display a substantial arrest of seedling growth and have elevated EIN3 levels, even in the absence of exogenous ethylene. Collectively, our results show that the SCF EBF1/EBF2-dependent ubiquitination and subsequent removal of EIN3 is critical not only for proper ethylene signaling but also for growth in plants.

Original languageEnglish
Pages (from-to)6803-6808
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number17
DOIs
Publication statusPublished - 2004 Apr 27
Externally publishedYes

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Ubiquitin-Protein Ligases
Arabidopsis
Cullin Proteins
Growth
F-Box Proteins
Ubiquitination
ethylene
Plant Physiological Phenomena
Reverse Genetics
Plant Development
Eukaryotic Cells
Ligases
Seedlings
Proteolysis
Hypersensitivity
Mutation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. / Gagne, Jennifer M.; Smalle, Jan; Gingerich, Derek J.; Walker, Joseph M.; Yoo, Sang-Dong; Yanagisawa, Shuichi; Vierstra, Richard D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 17, 27.04.2004, p. 6803-6808.

Research output: Contribution to journalArticle

Gagne, Jennifer M. ; Smalle, Jan ; Gingerich, Derek J. ; Walker, Joseph M. ; Yoo, Sang-Dong ; Yanagisawa, Shuichi ; Vierstra, Richard D. / Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protein ligases that repress ethylene action and promote growth by directing EIN3 degradation. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 17. pp. 6803-6808.
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