Arabidopsis GDSL lipase 2 plays a role in pathogen defense via negative regulation of auxin signaling

Dong Sook Lee, Bo Kyung Kim, Sun Jae Kwon, Hak Chul Jin, Ohkmae K. Park

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

GLIP1 was isolated previously from Arabidopsis, as a salicylic acid-responsive secreted GDSL lipase that functions in resistance to Alternaria brassicicola [I.S. Oh, A.R. Park, M.S. Bae, S.J. Kwon, Y.S. Kim, J.E. Lee, N.Y. Kang, S. Lee, H. Cheong, O.K. Park, Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola. Plant Cell 17 (2005) 2832-2847.]. To extend our knowledge of the roles played by GLIPs in Arabidopsis, we conducted functional studies of another family member, GLIP2. GLIP2 transcripts were expressed in young seedlings, as well as in the root and stem tissues of mature plants. GLIP2 transcript levels were elevated by treatment with salicylic acid, jasmonic acid and ethylene. Recombinant GLIP2 proteins possessed lipase and anti-microbial activities, inhibiting germination of fungal spores. In comparison to wild type plants, T-DNA insertion glip2 mutants exhibited enhanced auxin responses, including increased lateral root formation and elevated AUX/IAA gene expression. When challenged with the necrotropic bacteria Erwinia carotovora, glip2 mutants exhibited more susceptible phenotypes than wild type plants. These results suggest that GLIP2 plays a role in resistance to Erwinia carotovora via negative regulation of auxin signaling.

Original languageEnglish
Pages (from-to)1038-1042
Number of pages5
JournalBiochemical and biophysical research communications
Volume379
Issue number4
DOIs
Publication statusPublished - 2009 Feb 20

Keywords

  • Arabidopsis
  • Auxin signaling
  • Erwinia carotovora
  • GDSL lipase
  • GLIP2
  • Pathogen resistance

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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