Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression

Minsoo Noh, Jin Seok Shin, Jong Chan Hong, Soo Youn Kim, Jeong Sheop Shin

Research output: Contribution to journalArticlepeer-review

Abstract

Key message: TCX8 localizes to nucleus and has transcriptional repression activity. TCX8 binds to the promoter region of LOX2 encoding lipoxygenase, causing JA biosynthesis suppression, and thereby delays plant senescence. Abstract: Conserved CXC domain-containing proteins are found in most eukaryotes. Eight TCX proteins, which are homologs of animal CXC–Hinge–CXC (CHC) proteins, were identified in Arabidopsis, and three of them, TSO1, TCX2/SOL2 and TCX3/SOL1, have been reported to affect cell-cycle control. TCX8, one of the TCX family proteins, was believed to be a TF but its precise function has not been reported. Yeast two-hybrid screening revealed TCP20, a TF that binds to the promoter of LOX2 encoding lipoxygenase, as a strong candidate for interaction with TCX8. We confirmed that TCX8 directly interacts with TCP20 using in vitro pull-down assay and in vivo BiFC and observed that TCX8, as a TF, localizes to nucleus. Using EMSA and by analyzing phenotypes of TCX8-overexpression lines, we demonstrated that TCX8 regulates the expression of LOX2 by binding to either cis-element of LOX2 promoter to which TCP20 or TCP4 binds, affecting JA biosynthesis, and thereby delaying plant senescence. Our study provides new information about the role of TCX8 in modulating plant senescence through regulating LOX2 expression.

Original languageEnglish
Pages (from-to)677-689
Number of pages13
JournalPlant Cell Reports
Volume40
Issue number4
DOIs
Publication statusPublished - 2021 Apr

Keywords

  • Arabidopsis thaliana
  • LOX2
  • Senescence
  • TCP20
  • TCP4
  • TCX8

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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