Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein

Min Jung Kim, Hwa Ran Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The cucumber mosaic virus (CMV) replication complex has previously been shown to associate with cellular membranes. However, it remains unknown whether any host factors participate in this process. In this study, five groups of Arabidopsis tonoplast intrinsic protein (TIP) genes were isolated and the proteins they encoded were evaluated with regard to their interactions with CMV proteins. TIP1 and TIP2 were found to interact with the CMV 1a protein in the Sos recruitment system, whereas no interactions with the other three TIP subgroups were observed in this assay. The interaction of CMV 1a with the TIP1 and TIP2 proteins was confirmed via co-immunoprecipitation assays. Additionally, CMV 1a co-localized with TIP1 and TIP2 in transfected Arabidopsis protoplasts. The findings of this study suggest that members of two TIP subfamilies might affect CMV replication via interaction with CMV 1a in the tonoplasts.

Original languageEnglish
Pages (from-to)3425-3431
Number of pages7
JournalJournal of General Virology
Volume87
Issue number11
DOIs
Publication statusPublished - 2006 Nov 1

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Cucumovirus
Virus Replication
Proteins
Arabidopsis
Protoplasts
Immunoprecipitation
Arabidopsis TIP1 protein
cucumber mosaic virus 1a protein
Membranes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein. / Kim, Min Jung; Kim, Hwa Ran; Paek, Kyung Hee.

In: Journal of General Virology, Vol. 87, No. 11, 01.11.2006, p. 3425-3431.

Research output: Contribution to journalArticle

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