Arabidopsis UDP-glycosyltransferase 78D1-overexpressing plants accumulate higher levels of kaempferol 3-O-β-d-glucopyranoside than wild-type plants

Won Je Lee, Jaeho Kim, Dongho Lee, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Flavonols are a class of flavonoids that are found in most plants. Certain flavonols exhibit anticancer, antioxidant, and antimicrobial functions. An array of genes plays a role in the regulation of flavonoid biosynthetic pathways, including the MYB–bHLH (basic helix-loop-helix-WD40 transcription factor complex. Flavonoids often display altered bioactivities after being glycosylated by the action of glycosyltransferases. These enzymes catalyze the transfer of sugars from a donor to various acceptors. In this study, we generated several transgenic lines of Arabidopsis that overexpress UDP-glycosyltransferase 78D1 (UGT78D1), which are hereafter referred to as UGT78D1-OX, to address three questions: (1) Can UGT78D1-OX seedlings accumulate more flavonols? (2) Can UGT78D1-OX seedlings accumulate more flavonols in the presence of sucrose? and (3) Will UGT78D1-OX be more sensitive to abiotic stresses? We observed that UGT78D1-OX seedlings accumulated specific types of kaempferol, while they had a decreased content of flavonols in the presence of sucrose. Contrary to our expectation, more anthocyanins accumulated in the UGT78D1-OX lines, although the expression of production of anthocyanin pigment 1 was slightly reduced in UGT78D1-OX seedlings compared with that in wild-type seedlings. It appeared that the overexpression of UGT78D1 did not interfere with abiotic stress tolerance in the mutant plants.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalApplied Biological Chemistry
DOIs
Publication statusAccepted/In press - 2017 Oct 10

Fingerprint

Glycosyltransferases
Uridine Diphosphate
Arabidopsis
Flavonols
Seedlings
Flavonoids
Anthocyanins
Sucrose
kaempferol
Basic Helix-Loop-Helix Transcription Factors
Biosynthetic Pathways
Bioactivity
Pigments
Sugars
Transcription Factors
Antioxidants
Genes

Keywords

  • Anthocyanin
  • Arabidopsis
  • Flavonoid
  • Flavonol
  • UDP-glycosyltransferase 78D1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry

Cite this

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title = "Arabidopsis UDP-glycosyltransferase 78D1-overexpressing plants accumulate higher levels of kaempferol 3-O-β-d-glucopyranoside than wild-type plants",
abstract = "Flavonols are a class of flavonoids that are found in most plants. Certain flavonols exhibit anticancer, antioxidant, and antimicrobial functions. An array of genes plays a role in the regulation of flavonoid biosynthetic pathways, including the MYB–bHLH (basic helix-loop-helix-WD40 transcription factor complex. Flavonoids often display altered bioactivities after being glycosylated by the action of glycosyltransferases. These enzymes catalyze the transfer of sugars from a donor to various acceptors. In this study, we generated several transgenic lines of Arabidopsis that overexpress UDP-glycosyltransferase 78D1 (UGT78D1), which are hereafter referred to as UGT78D1-OX, to address three questions: (1) Can UGT78D1-OX seedlings accumulate more flavonols? (2) Can UGT78D1-OX seedlings accumulate more flavonols in the presence of sucrose? and (3) Will UGT78D1-OX be more sensitive to abiotic stresses? We observed that UGT78D1-OX seedlings accumulated specific types of kaempferol, while they had a decreased content of flavonols in the presence of sucrose. Contrary to our expectation, more anthocyanins accumulated in the UGT78D1-OX lines, although the expression of production of anthocyanin pigment 1 was slightly reduced in UGT78D1-OX seedlings compared with that in wild-type seedlings. It appeared that the overexpression of UGT78D1 did not interfere with abiotic stress tolerance in the mutant plants.",
keywords = "Anthocyanin, Arabidopsis, Flavonoid, Flavonol, UDP-glycosyltransferase 78D1",
author = "Lee, {Won Je} and Jaeho Kim and Dongho Lee and Hong, {Suk Whan} and Hojoung Lee",
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AU - Lee, Won Je

AU - Kim, Jaeho

AU - Lee, Dongho

AU - Hong, Suk Whan

AU - Lee, Hojoung

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N2 - Flavonols are a class of flavonoids that are found in most plants. Certain flavonols exhibit anticancer, antioxidant, and antimicrobial functions. An array of genes plays a role in the regulation of flavonoid biosynthetic pathways, including the MYB–bHLH (basic helix-loop-helix-WD40 transcription factor complex. Flavonoids often display altered bioactivities after being glycosylated by the action of glycosyltransferases. These enzymes catalyze the transfer of sugars from a donor to various acceptors. In this study, we generated several transgenic lines of Arabidopsis that overexpress UDP-glycosyltransferase 78D1 (UGT78D1), which are hereafter referred to as UGT78D1-OX, to address three questions: (1) Can UGT78D1-OX seedlings accumulate more flavonols? (2) Can UGT78D1-OX seedlings accumulate more flavonols in the presence of sucrose? and (3) Will UGT78D1-OX be more sensitive to abiotic stresses? We observed that UGT78D1-OX seedlings accumulated specific types of kaempferol, while they had a decreased content of flavonols in the presence of sucrose. Contrary to our expectation, more anthocyanins accumulated in the UGT78D1-OX lines, although the expression of production of anthocyanin pigment 1 was slightly reduced in UGT78D1-OX seedlings compared with that in wild-type seedlings. It appeared that the overexpression of UGT78D1 did not interfere with abiotic stress tolerance in the mutant plants.

AB - Flavonols are a class of flavonoids that are found in most plants. Certain flavonols exhibit anticancer, antioxidant, and antimicrobial functions. An array of genes plays a role in the regulation of flavonoid biosynthetic pathways, including the MYB–bHLH (basic helix-loop-helix-WD40 transcription factor complex. Flavonoids often display altered bioactivities after being glycosylated by the action of glycosyltransferases. These enzymes catalyze the transfer of sugars from a donor to various acceptors. In this study, we generated several transgenic lines of Arabidopsis that overexpress UDP-glycosyltransferase 78D1 (UGT78D1), which are hereafter referred to as UGT78D1-OX, to address three questions: (1) Can UGT78D1-OX seedlings accumulate more flavonols? (2) Can UGT78D1-OX seedlings accumulate more flavonols in the presence of sucrose? and (3) Will UGT78D1-OX be more sensitive to abiotic stresses? We observed that UGT78D1-OX seedlings accumulated specific types of kaempferol, while they had a decreased content of flavonols in the presence of sucrose. Contrary to our expectation, more anthocyanins accumulated in the UGT78D1-OX lines, although the expression of production of anthocyanin pigment 1 was slightly reduced in UGT78D1-OX seedlings compared with that in wild-type seedlings. It appeared that the overexpression of UGT78D1 did not interfere with abiotic stress tolerance in the mutant plants.

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