Arginine deiminase enhances dexamethasone-induced cytotoxicity in human T-lymphoblastic leukemia CCRF-CEM cells

Eun Joo Noh, Sang Wook Kang, Yong Jae Shin, Sang-Hyun Choi, Chan Gil Kim, In Sun Park, Denys N. Wheatley, Bon Hong Min

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Since arginine deiminase (ADI; EC 3.5.3.6) inhibits cell proliferation by arresting cells in the G 1 phase, we tested its synergistic effect on cell death induced by dexamethasone (DEX), which also induces apoptosis by G 1 cell cycle arrest. ADI inhibited cell proliferation and induced apoptosis in human leukemic CEM cells in a dose-dependent manner. Simultaneous treatment with ADI and DEX showed synergistic effects on DNA fragmentation and LDH release. In addition, ADI exerted its anti-proliferative activity against DEX-resistant CEH cells. ADI suppressed expression of c-myc, a potential key regulator of cell proliferation and apoptosis, and increased expression of p27 Kip1 cyclin-dependent kinase inhibitor. These results suggest that ADI efficiently increases the anti-cancer effect of DEX on human leukemic CEM cells through G 1 cell cycle arrest involving downregulation of c-myc and upregulation of p27 Kip1.

Original languageEnglish
Pages (from-to)502-508
Number of pages7
JournalInternational Journal of Cancer
Volume112
Issue number3
DOIs
Publication statusPublished - 2004 Nov 10

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
Gastrin-Secreting Cells
Dexamethasone
Cell Proliferation
Apoptosis
Cell Cycle Checkpoints
Cyclin-Dependent Kinase Inhibitor p27
DNA Fragmentation
Cell Death
Up-Regulation
Down-Regulation
arginine deiminase
Neoplasms

Keywords

  • Arginine
  • Arginine deiminase
  • c-myc
  • CEM leukemia cells
  • Dexamethasone
  • p27
  • Polyamine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Arginine deiminase enhances dexamethasone-induced cytotoxicity in human T-lymphoblastic leukemia CCRF-CEM cells. / Noh, Eun Joo; Kang, Sang Wook; Shin, Yong Jae; Choi, Sang-Hyun; Kim, Chan Gil; Park, In Sun; Wheatley, Denys N.; Min, Bon Hong.

In: International Journal of Cancer, Vol. 112, No. 3, 10.11.2004, p. 502-508.

Research output: Contribution to journalArticle

Noh, Eun Joo ; Kang, Sang Wook ; Shin, Yong Jae ; Choi, Sang-Hyun ; Kim, Chan Gil ; Park, In Sun ; Wheatley, Denys N. ; Min, Bon Hong. / Arginine deiminase enhances dexamethasone-induced cytotoxicity in human T-lymphoblastic leukemia CCRF-CEM cells. In: International Journal of Cancer. 2004 ; Vol. 112, No. 3. pp. 502-508.
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AU - Wheatley, Denys N.

AU - Min, Bon Hong

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