Artificial Chemical Reporter Targeting Strategy Using Bioorthogonal Click Reaction for Improving Active-Targeting Efficiency of Tumor

Hong Yeol Yoon, Min Lee Shin, Man Kyu Shim, Sangmin Lee, Jin Hee Na, Heebeom Koo, Hyukjin Lee, Jong Ho Kim, Kuen Yong Lee, Kwang Meyung Kim, Ick Chan Kwon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Biological ligands such as aptamer, antibody, glucose, and peptide have been widely used to bind specific surface molecules or receptors in tumor cells or subcellular structures to improve tumor-targeting efficiency of nanoparticles. However, this active-targeting strategy has limitations for tumor targeting due to inter- and intraheterogeneity of tumors. In this study, we demonstrated an alternative active-targeting strategy using metabolic engineering and bioorthogonal click reaction to improve tumor-targeting efficiency of nanoparticles. We observed that azide-containing chemical reporters were successfully generated onto surface glycans of various tumor cells such as lung cancer (A549), brain cancer (U87), and breast cancer (BT-474, MDA-MB231, MCF-7) via metabolic engineering in vitro. In addition, we compared tumor targeting of artificial azide reporter with bicyclononyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-CNPs) and integrin αvβ3 with cyclic RGD-conjugated CNPs (cRGD-CNPs) in vitro and in vivo. Fluorescence intensity of azide-reporter-targeted BCN-CNPs in tumor tissues was 1.6-fold higher and with a more uniform distribution compared to that of cRGD-CNPs. Moreover, even in the isolated heterogeneous U87 cells, BCN-CNPs could bind artificial azide reporters on tumor cells more uniformly (∼92.9%) compared to cRGD-CNPs. Therefore, the artificial azide-reporter-targeting strategy can be utilized for targeting heterogeneous tumor cells via bioorthogonal click reaction and may provide an alternative method of tumor targeting for further investigation in cancer therapy.

Original languageEnglish
Pages (from-to)1558-1570
Number of pages13
JournalMolecular Pharmaceutics
Volume14
Issue number5
DOIs
Publication statusPublished - 2017 May 1

Fingerprint

Azides
Neoplasms
Nanoparticles
Metabolic Engineering
Brain Neoplasms
Breast Neoplasms
Integrins
Polysaccharides
Lung Neoplasms
Fluorescence
Ligands
Glucose
Peptides
Antibodies
cyclic arginine-glycine-aspartic acid peptide

Keywords

  • active tumor targeting
  • bioorthogonal click reaction
  • heterogeneity
  • metabolic glycoengineering

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Artificial Chemical Reporter Targeting Strategy Using Bioorthogonal Click Reaction for Improving Active-Targeting Efficiency of Tumor. / Yoon, Hong Yeol; Shin, Min Lee; Shim, Man Kyu; Lee, Sangmin; Na, Jin Hee; Koo, Heebeom; Lee, Hyukjin; Kim, Jong Ho; Lee, Kuen Yong; Kim, Kwang Meyung; Kwon, Ick Chan.

In: Molecular Pharmaceutics, Vol. 14, No. 5, 01.05.2017, p. 1558-1570.

Research output: Contribution to journalArticle

Yoon, Hong Yeol ; Shin, Min Lee ; Shim, Man Kyu ; Lee, Sangmin ; Na, Jin Hee ; Koo, Heebeom ; Lee, Hyukjin ; Kim, Jong Ho ; Lee, Kuen Yong ; Kim, Kwang Meyung ; Kwon, Ick Chan. / Artificial Chemical Reporter Targeting Strategy Using Bioorthogonal Click Reaction for Improving Active-Targeting Efficiency of Tumor. In: Molecular Pharmaceutics. 2017 ; Vol. 14, No. 5. pp. 1558-1570.
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abstract = "Biological ligands such as aptamer, antibody, glucose, and peptide have been widely used to bind specific surface molecules or receptors in tumor cells or subcellular structures to improve tumor-targeting efficiency of nanoparticles. However, this active-targeting strategy has limitations for tumor targeting due to inter- and intraheterogeneity of tumors. In this study, we demonstrated an alternative active-targeting strategy using metabolic engineering and bioorthogonal click reaction to improve tumor-targeting efficiency of nanoparticles. We observed that azide-containing chemical reporters were successfully generated onto surface glycans of various tumor cells such as lung cancer (A549), brain cancer (U87), and breast cancer (BT-474, MDA-MB231, MCF-7) via metabolic engineering in vitro. In addition, we compared tumor targeting of artificial azide reporter with bicyclononyne (BCN)-conjugated glycol chitosan nanoparticles (BCN-CNPs) and integrin αvβ3 with cyclic RGD-conjugated CNPs (cRGD-CNPs) in vitro and in vivo. Fluorescence intensity of azide-reporter-targeted BCN-CNPs in tumor tissues was 1.6-fold higher and with a more uniform distribution compared to that of cRGD-CNPs. Moreover, even in the isolated heterogeneous U87 cells, BCN-CNPs could bind artificial azide reporters on tumor cells more uniformly (∼92.9{\%}) compared to cRGD-CNPs. Therefore, the artificial azide-reporter-targeting strategy can be utilized for targeting heterogeneous tumor cells via bioorthogonal click reaction and may provide an alternative method of tumor targeting for further investigation in cancer therapy.",
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AU - Yoon, Hong Yeol

AU - Shin, Min Lee

AU - Shim, Man Kyu

AU - Lee, Sangmin

AU - Na, Jin Hee

AU - Koo, Heebeom

AU - Lee, Hyukjin

AU - Kim, Jong Ho

AU - Lee, Kuen Yong

AU - Kim, Kwang Meyung

AU - Kwon, Ick Chan

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