Artificial transcription regulator as a tool for improvement of cellular property in Saccharomyces cerevisiae

Sang Woo Lee, Eunji Kim, Jin Soo Kim, Min-Kyu Oh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Modifying native organism through evolutionary engineering for desirable purpose often provides great breakthroughs to challenging obstacles in areas within biotechnology including biofuel production, screening of desirable phenotypes, etc. Conventional mutation methods, however, can be biased to certain types of mutations which restrict the accessibility to desirable phenotypes. In this study, we evaluated the potential of the library of synthetic zinc finger protein transcription factors (synthetic ZFP-TF library) for overcoming such limits. In the Saccharomyces cerevisiae sec14ts mutant background as a model eukaryotic system, we screened several sec14 bypass strains which can suppress growth defects through a malfunction of Sec14p with an introduced synthetic ZFP-TF library. Transcriptome analysis showed that many isolated strains exhibit simultaneous repression of genes which can suppress the growth defect. We also found that one of the isolated strains showed up-regulation of NTE1, which was not accessible by traditional evolutionary methods. These results showed that the synthetic ZFP-TF can affect multiple genes simultaneously and enables the activation of gene. Therefore, the synthetic ZFP-TF library can be a valuable tool to introduce global perturbations for phenotypic improvements and searching for unexplored phenotypic space to discover novel desirable phenotypes.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalChemical Engineering Science
Volume103
DOIs
Publication statusPublished - 2013 Nov 5

Fingerprint

Saccharomyces Cerevisiae
Transcription
Regulator
Yeast
Phenotype
Genes
Gene
Mutation
Defects
Biotechnology
Transcription factors
Biofuels
Zinc
Transcription Factor
Accessibility
Mutant
Biased
Screening
Activation
Transcription Factors

Keywords

  • Artificial transcription factor
  • Combinatorial approach
  • Phenotypic improvement
  • Saccharomyces cerevisiae
  • Synthetic biology
  • Zinc finger protein

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)
  • Applied Mathematics
  • Industrial and Manufacturing Engineering

Cite this

Artificial transcription regulator as a tool for improvement of cellular property in Saccharomyces cerevisiae. / Lee, Sang Woo; Kim, Eunji; Kim, Jin Soo; Oh, Min-Kyu.

In: Chemical Engineering Science, Vol. 103, 05.11.2013, p. 42-49.

Research output: Contribution to journalArticle

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