Asian dust storm particles induce a broad toxicological transcriptional program in human epidermal keratinocytes

Hyun Choi, Dong Wook Shin, Wonnyon Kim, Seong-Jae Doh, Soo Hwan Lee, Minsoo Noh

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Exposure to airborne dust particles originated from seasonal Asian dust storms in Chinese and Mongolian deserts results in increased incidence of a range of diseases including asthma, contact dermatitis and conjunctivitis. The areas affected by Asian dust particles extend from East China to the west coast of North America. In order to study toxicological mechanisms in human skin, we evaluated the effects of dust particles collected during Asian dust storms (Asian dust particles) on gene expression in human epidermal keratinocytes (HEK). In HEK, exposure to Asian dust particles significantly increased gene expressions of cytochrome P450 1A1 (CYP1A1), CYP1A2, and CYP1B1, which is an indication of aryl hydrocarbon receptor (AHR) activation. In addition, Asian dust particles increased gene transcription of the cytokines IL-6, IL-8, and GM-CSF, which have broad pro-inflammatory and immunomodulatory properties. Asian dust particles significantly up-regulated expression of caspase 14 in HEK, suggesting that Asian dust particles directly affect keratinocyte differentiation. We also demonstrated that protein extract of pollen, a material frequently adsorbed onto Asian dust particles, potentially contributes to the increased transcription of IL-6, CYP1A1, CYP1A2, and CYP1B1. Taken together, these studies suggest that Asian dust particles can exert toxicological effects on human skin through the activation of the cellular detoxification system, the production of pro-inflammatory and immunomodulatory cytokines, and changes in the expression of proteins essential in normal epidermal differentiation.

Original languageEnglish
Pages (from-to)92-99
Number of pages8
JournalToxicology Letters
Volume200
Issue number1-2
DOIs
Publication statusPublished - 2011 Jan 15

Fingerprint

Dust
Keratinocytes
Toxicology
Particles (particulate matter)
Cytochrome P-450 CYP1A2
Transcription
Gene expression
Cytochrome P-450 Enzyme System
Caspase 14
Interleukin-6
Skin
Chemical activation
Dermatitis
Cytokines
Gene Expression
Aryl Hydrocarbon Receptors
Detoxification
Conjunctivitis
Contact Dermatitis
Granulocyte-Macrophage Colony-Stimulating Factor

Keywords

  • Asian dust storm particles
  • Caspase 14
  • CYP1A1
  • GM-CSF
  • Human epidermal keratinocytes
  • IL-6

ASJC Scopus subject areas

  • Toxicology

Cite this

Asian dust storm particles induce a broad toxicological transcriptional program in human epidermal keratinocytes. / Choi, Hyun; Shin, Dong Wook; Kim, Wonnyon; Doh, Seong-Jae; Lee, Soo Hwan; Noh, Minsoo.

In: Toxicology Letters, Vol. 200, No. 1-2, 15.01.2011, p. 92-99.

Research output: Contribution to journalArticle

Choi, Hyun ; Shin, Dong Wook ; Kim, Wonnyon ; Doh, Seong-Jae ; Lee, Soo Hwan ; Noh, Minsoo. / Asian dust storm particles induce a broad toxicological transcriptional program in human epidermal keratinocytes. In: Toxicology Letters. 2011 ; Vol. 200, No. 1-2. pp. 92-99.
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