Asian plantain (Plantago asiatica) essential oils suppress 3-hydroxy-3-methylglutaryl-co-enzyme A reductase expression in vitro and in vivo and show hypocholesterolaemic properties in mice

Mi Ja Chung, Kuen Woo Park, Kyoung Heon Kim, Cheong Tae Kim, Jun Pill Baek, Kyong Hwan Bang, Young Mi Choi, Sung-Joon Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Asian plantain (Plantago asiatica) essential oil (PAEO) contains multiple bioactive compounds, but its potential effects on lipid metabolism have not been examined. PAEO was found to be mostly composed of oxygenated monoterpenes, with linalool as the major component (82.5%, w/w), measured using GC-MS. Incubation of 0-200 μg PAEO/ml with HepG2 cells for 24 h resulted in no significant toxicity. Incubation with 0.2 mg PAEO/ml altered the expression of LDL receptor (+83%; P<0.05) and 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase (-37%; P<0.05), as assessed using RT-PCR. LDL oxidation was markedly inhibited by PAEO treatment due to the prevalence of linalool compounds in PAEO. Oral administration of PAEO for 3 weeks in C57BL/6 mice significantly reduced plasma total cholesterol and TAG concentrations by 29 and 46%, respectively. The mRNA (+58%; P<0.05), but not protein, levels of the LDL receptor were significantly higher, whereas both mRNA and protein levels of HMG-CoA reductase were significantly lower (-46 and -11%, respectively; P<0.05) in the liver of PAEO-fed than of control mice. The mRNA levels of CYP7A1 were marginally reduced in HepG2 cells, but not in mouse liver after PAEO treatment. Thus, PAEO may have hypocholesterolaemic effects by altering the expression of HMG-CoA reductase. Reduced TAG and oxidised LDL may provide additional cardiovascular protective benefits.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalBritish Journal of Nutrition
Volume99
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

Plantago
Volatile Oils
Oxidoreductases
Enzymes
LDL Receptors
Hep G2 Cells
Messenger RNA
In Vitro Techniques
Monoterpenes
Liver
Inbred C57BL Mouse
Lipid Metabolism
Oral Administration
Proteins

Keywords

  • 3-Hydroxy-3-methyl-glutaryl-co-enzyme A reductase
  • Cholesterol
  • Essential oils
  • Low-density lipoprotein-receptor
  • Plantago asiatica
  • Triacylglycerols

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Asian plantain (Plantago asiatica) essential oils suppress 3-hydroxy-3-methylglutaryl-co-enzyme A reductase expression in vitro and in vivo and show hypocholesterolaemic properties in mice. / Chung, Mi Ja; Woo Park, Kuen; Kim, Kyoung Heon; Kim, Cheong Tae; Pill Baek, Jun; Bang, Kyong Hwan; Choi, Young Mi; Lee, Sung-Joon.

In: British Journal of Nutrition, Vol. 99, No. 1, 01.01.2008, p. 67-75.

Research output: Contribution to journalArticle

@article{f7b1720bd7324adbbc948e4c9048d56d,
title = "Asian plantain (Plantago asiatica) essential oils suppress 3-hydroxy-3-methylglutaryl-co-enzyme A reductase expression in vitro and in vivo and show hypocholesterolaemic properties in mice",
abstract = "Asian plantain (Plantago asiatica) essential oil (PAEO) contains multiple bioactive compounds, but its potential effects on lipid metabolism have not been examined. PAEO was found to be mostly composed of oxygenated monoterpenes, with linalool as the major component (82.5{\%}, w/w), measured using GC-MS. Incubation of 0-200 μg PAEO/ml with HepG2 cells for 24 h resulted in no significant toxicity. Incubation with 0.2 mg PAEO/ml altered the expression of LDL receptor (+83{\%}; P<0.05) and 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase (-37{\%}; P<0.05), as assessed using RT-PCR. LDL oxidation was markedly inhibited by PAEO treatment due to the prevalence of linalool compounds in PAEO. Oral administration of PAEO for 3 weeks in C57BL/6 mice significantly reduced plasma total cholesterol and TAG concentrations by 29 and 46{\%}, respectively. The mRNA (+58{\%}; P<0.05), but not protein, levels of the LDL receptor were significantly higher, whereas both mRNA and protein levels of HMG-CoA reductase were significantly lower (-46 and -11{\%}, respectively; P<0.05) in the liver of PAEO-fed than of control mice. The mRNA levels of CYP7A1 were marginally reduced in HepG2 cells, but not in mouse liver after PAEO treatment. Thus, PAEO may have hypocholesterolaemic effects by altering the expression of HMG-CoA reductase. Reduced TAG and oxidised LDL may provide additional cardiovascular protective benefits.",
keywords = "3-Hydroxy-3-methyl-glutaryl-co-enzyme A reductase, Cholesterol, Essential oils, Low-density lipoprotein-receptor, Plantago asiatica, Triacylglycerols",
author = "Chung, {Mi Ja} and {Woo Park}, Kuen and Kim, {Kyoung Heon} and Kim, {Cheong Tae} and {Pill Baek}, Jun and Bang, {Kyong Hwan} and Choi, {Young Mi} and Sung-Joon Lee",
year = "2008",
month = "1",
day = "1",
doi = "10.1017/S0007114507798926",
language = "English",
volume = "99",
pages = "67--75",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "1",

}

TY - JOUR

T1 - Asian plantain (Plantago asiatica) essential oils suppress 3-hydroxy-3-methylglutaryl-co-enzyme A reductase expression in vitro and in vivo and show hypocholesterolaemic properties in mice

AU - Chung, Mi Ja

AU - Woo Park, Kuen

AU - Kim, Kyoung Heon

AU - Kim, Cheong Tae

AU - Pill Baek, Jun

AU - Bang, Kyong Hwan

AU - Choi, Young Mi

AU - Lee, Sung-Joon

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Asian plantain (Plantago asiatica) essential oil (PAEO) contains multiple bioactive compounds, but its potential effects on lipid metabolism have not been examined. PAEO was found to be mostly composed of oxygenated monoterpenes, with linalool as the major component (82.5%, w/w), measured using GC-MS. Incubation of 0-200 μg PAEO/ml with HepG2 cells for 24 h resulted in no significant toxicity. Incubation with 0.2 mg PAEO/ml altered the expression of LDL receptor (+83%; P<0.05) and 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase (-37%; P<0.05), as assessed using RT-PCR. LDL oxidation was markedly inhibited by PAEO treatment due to the prevalence of linalool compounds in PAEO. Oral administration of PAEO for 3 weeks in C57BL/6 mice significantly reduced plasma total cholesterol and TAG concentrations by 29 and 46%, respectively. The mRNA (+58%; P<0.05), but not protein, levels of the LDL receptor were significantly higher, whereas both mRNA and protein levels of HMG-CoA reductase were significantly lower (-46 and -11%, respectively; P<0.05) in the liver of PAEO-fed than of control mice. The mRNA levels of CYP7A1 were marginally reduced in HepG2 cells, but not in mouse liver after PAEO treatment. Thus, PAEO may have hypocholesterolaemic effects by altering the expression of HMG-CoA reductase. Reduced TAG and oxidised LDL may provide additional cardiovascular protective benefits.

AB - Asian plantain (Plantago asiatica) essential oil (PAEO) contains multiple bioactive compounds, but its potential effects on lipid metabolism have not been examined. PAEO was found to be mostly composed of oxygenated monoterpenes, with linalool as the major component (82.5%, w/w), measured using GC-MS. Incubation of 0-200 μg PAEO/ml with HepG2 cells for 24 h resulted in no significant toxicity. Incubation with 0.2 mg PAEO/ml altered the expression of LDL receptor (+83%; P<0.05) and 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase (-37%; P<0.05), as assessed using RT-PCR. LDL oxidation was markedly inhibited by PAEO treatment due to the prevalence of linalool compounds in PAEO. Oral administration of PAEO for 3 weeks in C57BL/6 mice significantly reduced plasma total cholesterol and TAG concentrations by 29 and 46%, respectively. The mRNA (+58%; P<0.05), but not protein, levels of the LDL receptor were significantly higher, whereas both mRNA and protein levels of HMG-CoA reductase were significantly lower (-46 and -11%, respectively; P<0.05) in the liver of PAEO-fed than of control mice. The mRNA levels of CYP7A1 were marginally reduced in HepG2 cells, but not in mouse liver after PAEO treatment. Thus, PAEO may have hypocholesterolaemic effects by altering the expression of HMG-CoA reductase. Reduced TAG and oxidised LDL may provide additional cardiovascular protective benefits.

KW - 3-Hydroxy-3-methyl-glutaryl-co-enzyme A reductase

KW - Cholesterol

KW - Essential oils

KW - Low-density lipoprotein-receptor

KW - Plantago asiatica

KW - Triacylglycerols

UR - http://www.scopus.com/inward/record.url?scp=37349057813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37349057813&partnerID=8YFLogxK

U2 - 10.1017/S0007114507798926

DO - 10.1017/S0007114507798926

M3 - Article

C2 - 17697428

AN - SCOPUS:37349057813

VL - 99

SP - 67

EP - 75

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 1

ER -