Abstract
Objectives. Asia sand dust (ASD) is known to cause various human diseases including respiratory infection. The aim of this study was to examine the effect of ASD on inflammatory response in human middle ear epithelial cells (HMEECs) in vitro and in vivo. Methods. Cell viability was assessed using the cell counting kit-8 assay. The mRNA levels of various genes including COX2, TNF-α, MUC5AC, MUC5B, TP53, BAX, BCL-2, NOX4, and SOD1 were analyzed using semiquantitative realtime polymerase chain reaction. COX-2 protein levels were determined by western blot analysis. Sprague Dawley rats were used for in vivo investigations of inflammatory reactions in the middle ear epithelium as a result of ASD injection. Results. We observed dose-dependent decrease in HMEEC viability. ASD exposure significantly increased COX-2, TNF-α, MUC5AC, and MUC5B mRNA expression. Also, ASD affected the mRNA levels of apoptosis- and oxidative stressrelated genes. Western blot analysis revealed a dose-dependent increase in COX-2 production. Animal studies also demonstrated anASD-induced inflammatory response in the middle ear epithelium. Conclusion. Environmental ASD exposure can result in the development of otitis media.
| Original language | English |
|---|---|
| Pages (from-to) | 198-205 |
| Number of pages | 8 |
| Journal | Clinical and Experimental Otorhinolaryngology |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2016 Sep 1 |
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Keywords
- Asian Sand Dust
- Cyclooxygenase 2
- MUC5AC
- MUC5B
- Otitis Media
ASJC Scopus subject areas
- Surgery
- Otorhinolaryngology
Cite this
Asian sand dust enhances the inflammatory response and mucin gene expression in the middle ear. / Chang, Jiwon; Go, Yoonyoung; Park, Moo Kyun; Chae, Sungwon; Lee, Seon Heui; Song, Jae-Jun.
In: Clinical and Experimental Otorhinolaryngology, Vol. 9, No. 3, 01.09.2016, p. 198-205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Asian sand dust enhances the inflammatory response and mucin gene expression in the middle ear
AU - Chang, Jiwon
AU - Go, Yoonyoung
AU - Park, Moo Kyun
AU - Chae, Sungwon
AU - Lee, Seon Heui
AU - Song, Jae-Jun
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Objectives. Asia sand dust (ASD) is known to cause various human diseases including respiratory infection. The aim of this study was to examine the effect of ASD on inflammatory response in human middle ear epithelial cells (HMEECs) in vitro and in vivo. Methods. Cell viability was assessed using the cell counting kit-8 assay. The mRNA levels of various genes including COX2, TNF-α, MUC5AC, MUC5B, TP53, BAX, BCL-2, NOX4, and SOD1 were analyzed using semiquantitative realtime polymerase chain reaction. COX-2 protein levels were determined by western blot analysis. Sprague Dawley rats were used for in vivo investigations of inflammatory reactions in the middle ear epithelium as a result of ASD injection. Results. We observed dose-dependent decrease in HMEEC viability. ASD exposure significantly increased COX-2, TNF-α, MUC5AC, and MUC5B mRNA expression. Also, ASD affected the mRNA levels of apoptosis- and oxidative stressrelated genes. Western blot analysis revealed a dose-dependent increase in COX-2 production. Animal studies also demonstrated anASD-induced inflammatory response in the middle ear epithelium. Conclusion. Environmental ASD exposure can result in the development of otitis media.
AB - Objectives. Asia sand dust (ASD) is known to cause various human diseases including respiratory infection. The aim of this study was to examine the effect of ASD on inflammatory response in human middle ear epithelial cells (HMEECs) in vitro and in vivo. Methods. Cell viability was assessed using the cell counting kit-8 assay. The mRNA levels of various genes including COX2, TNF-α, MUC5AC, MUC5B, TP53, BAX, BCL-2, NOX4, and SOD1 were analyzed using semiquantitative realtime polymerase chain reaction. COX-2 protein levels were determined by western blot analysis. Sprague Dawley rats were used for in vivo investigations of inflammatory reactions in the middle ear epithelium as a result of ASD injection. Results. We observed dose-dependent decrease in HMEEC viability. ASD exposure significantly increased COX-2, TNF-α, MUC5AC, and MUC5B mRNA expression. Also, ASD affected the mRNA levels of apoptosis- and oxidative stressrelated genes. Western blot analysis revealed a dose-dependent increase in COX-2 production. Animal studies also demonstrated anASD-induced inflammatory response in the middle ear epithelium. Conclusion. Environmental ASD exposure can result in the development of otitis media.
KW - Asian Sand Dust
KW - Cyclooxygenase 2
KW - MUC5AC
KW - MUC5B
KW - Otitis Media
UR - http://www.scopus.com/inward/record.url?scp=84981539684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84981539684&partnerID=8YFLogxK
U2 - 10.21053/ceo.2015.01060
DO - 10.21053/ceo.2015.01060
M3 - Article
VL - 9
SP - 198
EP - 205
JO - Clinical and Experimental Otorhinolaryngology
JF - Clinical and Experimental Otorhinolaryngology
SN - 1976-8710
IS - 3
ER -