Aspergillus flavus expressed sequence tags and microarray as tools in understanding aflatoxin biosynthesis

J. Yu, T. E. Cleveland, J. R. Wilkinson, B. C. Campbell, J. H. Kim, Heenam Kim, D. Bhatnagar, G. A. Payne, W. C. Nierman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aflatoxins are the most toxic and carcinogenic naturally occurring mycotoxins. They are produced primarily by Aspergillus flavus and A. parasiticus. In order to better understand the molecular mechanisms that control aflatoxin production, identification of genes using A. flavus expressed sequence tags (ESTs) and microarrays is currently being performed. Sequencing and annotation of A. flavus ESTs from a normalized A. flavus cDNA library identified 7,218 unique EST sequences. Genes that are putatively involved in aflatoxin biosynthesis, regulation and signal transduction, fungal virulence or pathogenicity, stress response or antioxidation, and fungal development were identified from these ESTs. Microarrays containing over 5,000 unique A. flavus gene amplicons were constructed at The Institute for Genomic Research. Gene expression profiling under aflatoxin-producing and non-producing conditions using this microarray has identified hundreds of genes that are potentially involved in aflatoxin production. Further investigations on the functions of these genes by gene knockout experiments are underway. This research is expected to provide information for developing new strategies for controlling aflatoxin contamination of agricultural commodities.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalMycotoxin Research
Volume22
Issue number1
DOIs
Publication statusPublished - 2006 Mar 1
Externally publishedYes

Fingerprint

Aspergillus flavus
Aflatoxins
Aspergillus
Biosynthesis
Expressed Sequence Tags
Microarrays
Genes
Virulence
Signal transduction
Gene Knockout Techniques
Mycotoxins
Poisons
Gene Expression Profiling
Gene Library
Gene expression
Signal Transduction
Contamination
Research

Keywords

  • Aflatoxin biosynthesis
  • Aspergillus flavus
  • Genomics
  • Microarray
  • Mycotoxin
  • Pathogenesis

ASJC Scopus subject areas

  • Microbiology
  • Toxicology
  • Biotechnology

Cite this

Yu, J., Cleveland, T. E., Wilkinson, J. R., Campbell, B. C., Kim, J. H., Kim, H., ... Nierman, W. C. (2006). Aspergillus flavus expressed sequence tags and microarray as tools in understanding aflatoxin biosynthesis. Mycotoxin Research, 22(1), 16-21. https://doi.org/10.1007/BF02954552

Aspergillus flavus expressed sequence tags and microarray as tools in understanding aflatoxin biosynthesis. / Yu, J.; Cleveland, T. E.; Wilkinson, J. R.; Campbell, B. C.; Kim, J. H.; Kim, Heenam; Bhatnagar, D.; Payne, G. A.; Nierman, W. C.

In: Mycotoxin Research, Vol. 22, No. 1, 01.03.2006, p. 16-21.

Research output: Contribution to journalArticle

Yu, J, Cleveland, TE, Wilkinson, JR, Campbell, BC, Kim, JH, Kim, H, Bhatnagar, D, Payne, GA & Nierman, WC 2006, 'Aspergillus flavus expressed sequence tags and microarray as tools in understanding aflatoxin biosynthesis', Mycotoxin Research, vol. 22, no. 1, pp. 16-21. https://doi.org/10.1007/BF02954552
Yu, J. ; Cleveland, T. E. ; Wilkinson, J. R. ; Campbell, B. C. ; Kim, J. H. ; Kim, Heenam ; Bhatnagar, D. ; Payne, G. A. ; Nierman, W. C. / Aspergillus flavus expressed sequence tags and microarray as tools in understanding aflatoxin biosynthesis. In: Mycotoxin Research. 2006 ; Vol. 22, No. 1. pp. 16-21.
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