Aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats

Mingeum Jeong, Dong Sung Lee, Hyung Joo Suh, Yooheon Park

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Soybeans have been widely used to alleviate climacteric symptoms. In this study, we investigated the oestrogenic activities of isoflavones extracted from Aspergillus oryzae-challenged germinated soybeans (AO-GS). Eight-week-old virgin Sprague-Dawley female rats were ovariectomised (OVX). The rats were orally administered 0.1 mg kg-1 17α-ethinyl oestradiol or three different doses of AO-GS (0.5, 1.0 2.0 g kg-1 day-1) in distilled water for 6 weeks, while control rats were administered vehicle alone. Uterine weights and levels of oestradiol and testosterone in serum were measured. In addition to serum parameters, bone parameters were also acquired by using micro-computed tomography scanning. RESULTS: Treatments of OVX rats with AO-GS changed the secretory profile of serum oestradiol and testosterone. Serum oestradiol levels were significantly increased in OVX rats treated with and AO-GS (0.5, 1.0, 2.0 g kg-1 day-1), while serum testosterone levels were not significantly increased in OVX rats treated with 1.0 g kg-1 day-1 of AO-GS. Furthermore, AO-GS (2.0 g kg-1 day-1) significantly attenuated bone loss, increased trabecular bone volume fraction and trabecular thickness, and significantly decreased trabecular pattern factor. CONCLUSION: AO-GS treatments caused moderate oestrogenic activity in OVX rats compared to those treated with oestradiol, suggesting the potential for the use of AO-GS in the treatment of menopausal symptoms and in osteoporosis caused by oestrogen deficiency.

Original languageEnglish
Pages (from-to)979-987
Number of pages9
JournalJournal of the Science of Food and Agriculture
Volume96
Issue number3
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Aspergillus oryzae
Soybeans
signs and symptoms (animals and humans)
soybeans
rats
extracts
estradiol
Estradiol
testosterone
Testosterone
Serum
bones
micro-computed tomography
Bone and Bones
menopause
Ethinyl Estradiol
Isoflavones
osteoporosis
isoflavones
Osteoporosis

Keywords

  • Aspergillus oryzae
  • Micro-computed tomography (CT)
  • Ovariectomised rats
  • Soybeans

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Nutrition and Dietetics
  • Biotechnology

Cite this

Aspergillus oryzae fermented germinated soybean extract alleviates perimenopausal symptoms in ovariectomised rats. / Jeong, Mingeum; Lee, Dong Sung; Suh, Hyung Joo; Park, Yooheon.

In: Journal of the Science of Food and Agriculture, Vol. 96, No. 3, 01.02.2016, p. 979-987.

Research output: Contribution to journalArticle

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N2 - BACKGROUND: Soybeans have been widely used to alleviate climacteric symptoms. In this study, we investigated the oestrogenic activities of isoflavones extracted from Aspergillus oryzae-challenged germinated soybeans (AO-GS). Eight-week-old virgin Sprague-Dawley female rats were ovariectomised (OVX). The rats were orally administered 0.1 mg kg-1 17α-ethinyl oestradiol or three different doses of AO-GS (0.5, 1.0 2.0 g kg-1 day-1) in distilled water for 6 weeks, while control rats were administered vehicle alone. Uterine weights and levels of oestradiol and testosterone in serum were measured. In addition to serum parameters, bone parameters were also acquired by using micro-computed tomography scanning. RESULTS: Treatments of OVX rats with AO-GS changed the secretory profile of serum oestradiol and testosterone. Serum oestradiol levels were significantly increased in OVX rats treated with and AO-GS (0.5, 1.0, 2.0 g kg-1 day-1), while serum testosterone levels were not significantly increased in OVX rats treated with 1.0 g kg-1 day-1 of AO-GS. Furthermore, AO-GS (2.0 g kg-1 day-1) significantly attenuated bone loss, increased trabecular bone volume fraction and trabecular thickness, and significantly decreased trabecular pattern factor. CONCLUSION: AO-GS treatments caused moderate oestrogenic activity in OVX rats compared to those treated with oestradiol, suggesting the potential for the use of AO-GS in the treatment of menopausal symptoms and in osteoporosis caused by oestrogen deficiency.

AB - BACKGROUND: Soybeans have been widely used to alleviate climacteric symptoms. In this study, we investigated the oestrogenic activities of isoflavones extracted from Aspergillus oryzae-challenged germinated soybeans (AO-GS). Eight-week-old virgin Sprague-Dawley female rats were ovariectomised (OVX). The rats were orally administered 0.1 mg kg-1 17α-ethinyl oestradiol or three different doses of AO-GS (0.5, 1.0 2.0 g kg-1 day-1) in distilled water for 6 weeks, while control rats were administered vehicle alone. Uterine weights and levels of oestradiol and testosterone in serum were measured. In addition to serum parameters, bone parameters were also acquired by using micro-computed tomography scanning. RESULTS: Treatments of OVX rats with AO-GS changed the secretory profile of serum oestradiol and testosterone. Serum oestradiol levels were significantly increased in OVX rats treated with and AO-GS (0.5, 1.0, 2.0 g kg-1 day-1), while serum testosterone levels were not significantly increased in OVX rats treated with 1.0 g kg-1 day-1 of AO-GS. Furthermore, AO-GS (2.0 g kg-1 day-1) significantly attenuated bone loss, increased trabecular bone volume fraction and trabecular thickness, and significantly decreased trabecular pattern factor. CONCLUSION: AO-GS treatments caused moderate oestrogenic activity in OVX rats compared to those treated with oestradiol, suggesting the potential for the use of AO-GS in the treatment of menopausal symptoms and in osteoporosis caused by oestrogen deficiency.

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