Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Chung Chieh Yu, Condon Lau, James W. Tunnell, Martin Hunter, Maxim Kalashnikov, Christopher Fang-Yen, Stephen F. Fulghum, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

Original languageEnglish
Pages (from-to)3119-3121
Number of pages3
JournalOptics Letters
Volume31
Issue number21
DOIs
Publication statusPublished - 2006 Nov 1
Externally publishedYes

Fingerprint

Spectrum Analysis
light scattering
Epithelial Cells
Light
Cell Nucleus
Organelles
spectroscopy
large-scale structure of the universe
Colon
organelles
backscattering
cancer
asymmetry
Neoplasms
nuclei
cells
scattering

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Yu, C. C., Lau, C., Tunnell, J. W., Hunter, M., Kalashnikov, M., Fang-Yen, C., ... Feld, M. S. (2006). Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy. Optics Letters, 31(21), 3119-3121. https://doi.org/10.1364/OL.31.003119

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy. / Yu, Chung Chieh; Lau, Condon; Tunnell, James W.; Hunter, Martin; Kalashnikov, Maxim; Fang-Yen, Christopher; Fulghum, Stephen F.; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.

In: Optics Letters, Vol. 31, No. 21, 01.11.2006, p. 3119-3121.

Research output: Contribution to journalArticle

Yu, CC, Lau, C, Tunnell, JW, Hunter, M, Kalashnikov, M, Fang-Yen, C, Fulghum, SF, Badizadegan, K, Dasari, RR & Feld, MS 2006, 'Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy', Optics Letters, vol. 31, no. 21, pp. 3119-3121. https://doi.org/10.1364/OL.31.003119
Yu CC, Lau C, Tunnell JW, Hunter M, Kalashnikov M, Fang-Yen C et al. Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy. Optics Letters. 2006 Nov 1;31(21):3119-3121. https://doi.org/10.1364/OL.31.003119
Yu, Chung Chieh ; Lau, Condon ; Tunnell, James W. ; Hunter, Martin ; Kalashnikov, Maxim ; Fang-Yen, Christopher ; Fulghum, Stephen F. ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. / Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy. In: Optics Letters. 2006 ; Vol. 31, No. 21. pp. 3119-3121.
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