Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Chung Chieh Yu, Condon Lau, James W. Tunnell, Martin Hunter, Maxim Kalashnikov, Christopher Fang-Yen, Stephen F. Fulghum, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

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25 Citations (Scopus)

Abstract

We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues.

Original languageEnglish
Pages (from-to)3119-3121
Number of pages3
JournalOptics Letters
Volume31
Issue number21
DOIs
Publication statusPublished - 2006 Nov 1

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Yu, C. C., Lau, C., Tunnell, J. W., Hunter, M., Kalashnikov, M., Fang-Yen, C., Fulghum, S. F., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2006). Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy. Optics Letters, 31(21), 3119-3121. https://doi.org/10.1364/OL.31.003119