Assessment of benzene, toluene, ethyl-benzene, and xylene (BTEX) toxicity in soil using sulfur-oxidizing bacterial (SOB) bioassay

Naveed Ahmed, Yong Sik Ok, Byong Hun Jeon, Jung Rae Kim, Kyu Jung Chae, Sang Eun Oh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The assessment of benzene, toluene, ethyl-benzene, and xylene (BTEX)-contaminated soil toxicity was performed using a sulfur-oxidizing bacteria (SOB) assay. The experiments were set up using an individual pollutant in a 25-mL bottle sealed with a rubber stopper and aluminum cap since BTEX are volatile. A large headspace volume (14 mL) was kept in the reactors to provide enough oxygen for the SOB. Soil samples were spiked with BTEX compounds in the concentration range of 1–1000 mg/kg. In reactors without BTEX compounds, approximately 85% of the theoretically required oxygen was consumed. Whereas, the reactors with benzene consumed in the range of 82–64% (5–100 mg/kg), those with toluene consumed 76–53% (1–50 mg/kg), those with ethyl-benzene consumed 44–71% (5–100 mg/kg), and those with xylene consumed 64–71% (1–10 mg/kg) of the theoretically required oxygen. The effective concentrations responsible for 50% growth inhibition (EC50) for benzene, toluene, ethyl-benzene, and xylene detection were 130.2, 1.2, 15.2, and 0.7 mg/kg, respectively. These results suggest that this SOB-based bioassay can detect BTEX pollutants in soils.

Original languageEnglish
Pages (from-to)651-657
Number of pages7
JournalChemosphere
Volume220
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Xylenes
Ethylbenzene
BTEX
Bioassay
Toluene
Xylene
Benzene
Sulfur
Biological Assay
Toxicity
bioassay
Soil
sulfur
toxicity
Soils
Container closures
soil
oxygen
benzene
Oxygen

Keywords

  • Benzene
  • BTEX toxicity
  • Ethyl-benzene
  • Soil toxicity
  • Sulfur-oxidizing bacterial bioassay
  • Toluene
  • Xylene

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Assessment of benzene, toluene, ethyl-benzene, and xylene (BTEX) toxicity in soil using sulfur-oxidizing bacterial (SOB) bioassay. / Ahmed, Naveed; Ok, Yong Sik; Jeon, Byong Hun; Kim, Jung Rae; Chae, Kyu Jung; Oh, Sang Eun.

In: Chemosphere, Vol. 220, 01.04.2019, p. 651-657.

Research output: Contribution to journalArticle

Ahmed, Naveed ; Ok, Yong Sik ; Jeon, Byong Hun ; Kim, Jung Rae ; Chae, Kyu Jung ; Oh, Sang Eun. / Assessment of benzene, toluene, ethyl-benzene, and xylene (BTEX) toxicity in soil using sulfur-oxidizing bacterial (SOB) bioassay. In: Chemosphere. 2019 ; Vol. 220. pp. 651-657.
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