Assessment of collagen-induced arthritis using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles

Correlation with 18F-Fluorodeoxyglucose positron emission tomography data

Ji Hyeon Cha, Sang Hoon Lee, Sheen Woo Lee, Kyeongsoon Park, Dae Hyuk Moon, Kwang Meyung Kim, Sandip Biswal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). Materials and Methods: We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 μL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice. Results: The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 ± 0.76 and 0.79 ± 0.71, respectively, for CIA mice; and 0.57 ± 0.17 and 0.54 ± 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 ± 1.54 x 105 and 2.75 ± 1.51 x 105, respectively, for CIA mice, and 1.22 ± 0.27 x 105 and 0.88 ± 0.24 x 105, respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice. Conclusion: NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.

Original languageEnglish
Pages (from-to)450-457
Number of pages8
JournalKorean journal of radiology
Volume13
Issue number4
DOIs
Publication statusPublished - 2012 Aug 1
Externally publishedYes

Fingerprint

Experimental Arthritis
Fluorodeoxyglucose F18
Positron-Emission Tomography
Nanoparticles
Ankle
Optical Imaging
Knee
Intravenous Injections
glycol-chitosan
Photons
Rheumatoid Arthritis
Collagen
Fluorescence
CY5.5 cyanine dye

Keywords

  • F-FDG PET
  • HGC-Cy5.5
  • Near-infrared fluorescence imaging
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Assessment of collagen-induced arthritis using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles : Correlation with 18F-Fluorodeoxyglucose positron emission tomography data. / Cha, Ji Hyeon; Lee, Sang Hoon; Lee, Sheen Woo; Park, Kyeongsoon; Moon, Dae Hyuk; Kim, Kwang Meyung; Biswal, Sandip.

In: Korean journal of radiology, Vol. 13, No. 4, 01.08.2012, p. 450-457.

Research output: Contribution to journalArticle

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title = "Assessment of collagen-induced arthritis using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles: Correlation with 18F-Fluorodeoxyglucose positron emission tomography data",
abstract = "Objective: To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). Materials and Methods: We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 μL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice. Results: The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 ± 0.76 and 0.79 ± 0.71, respectively, for CIA mice; and 0.57 ± 0.17 and 0.54 ± 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 ± 1.54 x 105 and 2.75 ± 1.51 x 105, respectively, for CIA mice, and 1.22 ± 0.27 x 105 and 0.88 ± 0.24 x 105, respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice. Conclusion: NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.",
keywords = "F-FDG PET, HGC-Cy5.5, Near-infrared fluorescence imaging, Rheumatoid arthritis",
author = "Cha, {Ji Hyeon} and Lee, {Sang Hoon} and Lee, {Sheen Woo} and Kyeongsoon Park and Moon, {Dae Hyuk} and Kim, {Kwang Meyung} and Sandip Biswal",
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T1 - Assessment of collagen-induced arthritis using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles

T2 - Correlation with 18F-Fluorodeoxyglucose positron emission tomography data

AU - Cha, Ji Hyeon

AU - Lee, Sang Hoon

AU - Lee, Sheen Woo

AU - Park, Kyeongsoon

AU - Moon, Dae Hyuk

AU - Kim, Kwang Meyung

AU - Biswal, Sandip

PY - 2012/8/1

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N2 - Objective: To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). Materials and Methods: We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 μL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice. Results: The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 ± 0.76 and 0.79 ± 0.71, respectively, for CIA mice; and 0.57 ± 0.17 and 0.54 ± 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 ± 1.54 x 105 and 2.75 ± 1.51 x 105, respectively, for CIA mice, and 1.22 ± 0.27 x 105 and 0.88 ± 0.24 x 105, respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice. Conclusion: NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.

AB - Objective: To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). Materials and Methods: We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 μL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice. Results: The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 ± 0.76 and 0.79 ± 0.71, respectively, for CIA mice; and 0.57 ± 0.17 and 0.54 ± 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 ± 1.54 x 105 and 2.75 ± 1.51 x 105, respectively, for CIA mice, and 1.22 ± 0.27 x 105 and 0.88 ± 0.24 x 105, respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice. Conclusion: NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.

KW - F-FDG PET

KW - HGC-Cy5.5

KW - Near-infrared fluorescence imaging

KW - Rheumatoid arthritis

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