Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

J. Rhee; Eunjung Han; Kuk Jin Nam; Kang Hyeon Lim; Yoon Chan Rah; S. Park; Soonil Koun; Hae Chul Park; June Choi

doi:10.1016/j.ijporl.2019.109611

Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

J. Rhee, Eunjung Han, Kuk Jin Nam, Kang Hyeon Lim, Yoon Chan Rah, S. Park, Soonil Koun, Hae Chul Park, June Choi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objectives: Particulate matter (PM) exposure has become one of the most serious problems. The aim of the present study was to evaluate the hair cell damage and possible developmental toxicity caused by PM_2.5 exposure using a zebrafish model. Methods: Zebrafish embryos were exposed to various concentrations of PM_2.5. Developmental toxicity was evaluated based on general morphology score (GMS) system and Panzica-Kelly score, and by measurement of body length and heart rate. To evaluate hair cell damage, the average number of total hair cells within four neuromasts exposed to various concentrations of PM_2.5 was compared with that of the control group. Results: Morphological abnormalities evaluated by the GMS system and Panzica-Kelly score were rare and body length tended to be shorter in the PM_2.5-exposed groups. Heart rate decreased significantly in the PM_2.5-exposed group. Additionally, significant hair cell damage was observed after PM_2.5 exposure. It was dose-dependent and more severe after a longer period exposure (10 dpf). Conclusions: In zebrafish embryos, exposure of PM_2.5 in the early stages of life decreased heart rate and caused significant hair cell damage in a dose-dependent manner.

Original language	English
Article number	109611
Journal	International Journal of Pediatric Otorhinolaryngology
Volume	126
DOIs	https://doi.org/10.1016/j.ijporl.2019.109611
Publication status	Published - 2019 Nov

Keywords

Hair cell
Particulate matter
Toxicity
Zebrafish

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Otorhinolaryngology

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10.1016/j.ijporl.2019.109611

Cite this

Rhee, J., Han, E., Nam, K. J., Lim, K. H., Chan Rah, Y., Park, S., Koun, S., Park, H. C., & Choi, J. (2019). Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models. International Journal of Pediatric Otorhinolaryngology, 126, [109611]. https://doi.org/10.1016/j.ijporl.2019.109611

@article{db33db677e8043678316ac964794beb3,

title = "Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models",

abstract = "Objectives: Particulate matter (PM) exposure has become one of the most serious problems. The aim of the present study was to evaluate the hair cell damage and possible developmental toxicity caused by PM2.5 exposure using a zebrafish model. Methods: Zebrafish embryos were exposed to various concentrations of PM2.5. Developmental toxicity was evaluated based on general morphology score (GMS) system and Panzica-Kelly score, and by measurement of body length and heart rate. To evaluate hair cell damage, the average number of total hair cells within four neuromasts exposed to various concentrations of PM2.5 was compared with that of the control group. Results: Morphological abnormalities evaluated by the GMS system and Panzica-Kelly score were rare and body length tended to be shorter in the PM2.5-exposed groups. Heart rate decreased significantly in the PM2.5-exposed group. Additionally, significant hair cell damage was observed after PM2.5 exposure. It was dose-dependent and more severe after a longer period exposure (10 dpf). Conclusions: In zebrafish embryos, exposure of PM2.5 in the early stages of life decreased heart rate and caused significant hair cell damage in a dose-dependent manner.",

keywords = "Hair cell, Particulate matter, Toxicity, Zebrafish",

author = "J. Rhee and Eunjung Han and Nam, {Kuk Jin} and Lim, {Kang Hyeon} and {Chan Rah}, Yoon and S. Park and Soonil Koun and Park, {Hae Chul} and June Choi",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (grant number 2017R1D1A1B03031860 ) and Korea University Future Research Grant. Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (grant number 2017R1D1A1B03031860) and Korea University Future Research Grant. The authors are grateful to Dr. Hee Jae Shin at the Korea Institute of Ocean Science & Technology, KIOST) and Hyeon Cheol Jeong of the facility management team at Korea University Ansan Hospital for assistance with the collection and extraction of the fine particulate matter. Especially we are grateful to my brothers, Dr. Seung Hyun Cho and Eun Joong Kim at Soo ENT Clinic. Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = nov,

doi = "10.1016/j.ijporl.2019.109611",

language = "English",

volume = "126",

journal = "International Journal of Pediatric Otorhinolaryngology",

issn = "0165-5876",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

AU - Rhee, J.

AU - Han, Eunjung

AU - Nam, Kuk Jin

AU - Lim, Kang Hyeon

AU - Chan Rah, Yoon

AU - Park, S.

AU - Koun, Soonil

AU - Park, Hae Chul

AU - Choi, June

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (grant number 2017R1D1A1B03031860 ) and Korea University Future Research Grant. Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (grant number 2017R1D1A1B03031860) and Korea University Future Research Grant. The authors are grateful to Dr. Hee Jae Shin at the Korea Institute of Ocean Science & Technology, KIOST) and Hyeon Cheol Jeong of the facility management team at Korea University Ansan Hospital for assistance with the collection and extraction of the fine particulate matter. Especially we are grateful to my brothers, Dr. Seung Hyun Cho and Eun Joong Kim at Soo ENT Clinic. Publisher Copyright: © 2019 Elsevier B.V.

PY - 2019/11

Y1 - 2019/11

N2 - Objectives: Particulate matter (PM) exposure has become one of the most serious problems. The aim of the present study was to evaluate the hair cell damage and possible developmental toxicity caused by PM2.5 exposure using a zebrafish model. Methods: Zebrafish embryos were exposed to various concentrations of PM2.5. Developmental toxicity was evaluated based on general morphology score (GMS) system and Panzica-Kelly score, and by measurement of body length and heart rate. To evaluate hair cell damage, the average number of total hair cells within four neuromasts exposed to various concentrations of PM2.5 was compared with that of the control group. Results: Morphological abnormalities evaluated by the GMS system and Panzica-Kelly score were rare and body length tended to be shorter in the PM2.5-exposed groups. Heart rate decreased significantly in the PM2.5-exposed group. Additionally, significant hair cell damage was observed after PM2.5 exposure. It was dose-dependent and more severe after a longer period exposure (10 dpf). Conclusions: In zebrafish embryos, exposure of PM2.5 in the early stages of life decreased heart rate and caused significant hair cell damage in a dose-dependent manner.

AB - Objectives: Particulate matter (PM) exposure has become one of the most serious problems. The aim of the present study was to evaluate the hair cell damage and possible developmental toxicity caused by PM2.5 exposure using a zebrafish model. Methods: Zebrafish embryos were exposed to various concentrations of PM2.5. Developmental toxicity was evaluated based on general morphology score (GMS) system and Panzica-Kelly score, and by measurement of body length and heart rate. To evaluate hair cell damage, the average number of total hair cells within four neuromasts exposed to various concentrations of PM2.5 was compared with that of the control group. Results: Morphological abnormalities evaluated by the GMS system and Panzica-Kelly score were rare and body length tended to be shorter in the PM2.5-exposed groups. Heart rate decreased significantly in the PM2.5-exposed group. Additionally, significant hair cell damage was observed after PM2.5 exposure. It was dose-dependent and more severe after a longer period exposure (10 dpf). Conclusions: In zebrafish embryos, exposure of PM2.5 in the early stages of life decreased heart rate and caused significant hair cell damage in a dose-dependent manner.

KW - Hair cell

KW - Particulate matter

KW - Toxicity

KW - Zebrafish

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U2 - 10.1016/j.ijporl.2019.109611

DO - 10.1016/j.ijporl.2019.109611

M3 - Article

C2 - 31374386

AN - SCOPUS:85069929473

SN - 0165-5876

VL - 126

JO - International Journal of Pediatric Otorhinolaryngology

JF - International Journal of Pediatric Otorhinolaryngology

M1 - 109611

ER -

Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

Abstract

Keywords

ASJC Scopus subject areas

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Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this

Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5 μm (PM 2.5) exposure using zebrafish (Danio rerio) models