Assessment of phototoxicity, skin irritation, and sensitization potential of polystyrene and TiO2 nanoparticles

Yoon Hee Park, Sang Hoon Jeong, Sang Min Yi, Byeong Hyeok Choi, Yu Ri Kim, In Kyoung Kim, Meyoung Kon Kim, And Sang Wook Son

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The human skin equivalent model (HSEM) is well known as an attractive alternative model for evaluation of dermal toxicity. However, only limited data are available on the usefulness of an HSEM for nanotoxicity testing. This study was designed to investigate cutaneous toxicity of polystyrene and TiO 2 nanoparticles using cultured keratinocytes, an HSEM, and an animal model. In addition, we also evaluated the skin sensitization potential of nanoparticles using a local lymph node assay with incorporation of BrdU. Findings from the present study indicate that polystyrene and TiO2 nanoparticles do not induce phototoxicity, acute cutaneous irritation, or skin sensitization. Results from evaluation of the HSEMs correspond well with those from animal models. Our findings suggest that the HSEM might be a useful alternative model for evaluation of dermal nanotoxicity.

Original languageEnglish
Article number012050
JournalJournal of Physics: Conference Series
Issue number1
Publication statusPublished - 2011 Jan 1


ASJC Scopus subject areas

  • Physics and Astronomy(all)

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