Assessment of the purity of emodin by quantitative nuclear magnetic resonance spectroscopy and mass balance

Sojung Park, Yu Jin Choi, Giang Hoang Do, Eun Kyoung Seo, Seunghun Hyun, Dongho Lee

Research output: Contribution to journalArticle

Abstract

Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalNatural Product Sciences
Volume25
Issue number3
DOIs
Publication statusPublished - 2019

Keywords

  • Emodin
  • HPLC-UV
  • Mass balance
  • QNMR

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry

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