Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population

Seung Zhoo Yoon, In Jin Jang, Yoon Ji Choi, Mae Hwa Kang, Hye Ja Lim, Young Jin Lim, Hye Won Lee, Seong Ho Chang, Suk Min Yoon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives: The G-308A polymorphism of the tumor necrosis factor α (TNF-α) gene has been suggested to be linked to high TNF promoter activity in in vitro studies. However, there have been some controversies in in vivo studies. This study investigated whether A allele at TNF-308 site is associated with (1) the changes in plasma cytokine levels during and after cardiopulmonary bypass (CPB) and (2) an increased incidence of pulmonary morbidity after CPB. Design: Prospective and observational investigation. Setting: A university hospital, single institution. Participants: Patients scheduled for cardiac surgery with CPB. Intervention: TNF genotype was determined by the real-time polymerase chain reaction method. IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay at the following time points: T1, before initiation of CPB; T2, 30 minutes of CPB; T3, 30 minutes after CPB; T4, 2 hours after CPB; and T5, 24 hours after CPB. The oxygen index, serum creatinine level, 24-hour blood loss, intubation time, and length of intensive care unit (ICU) stay were examined. Measurements and Main Results: The levels of TNF-α in group A (TNF-308GA/AA, n = 25) were higher at T3, T4, and T5 than group G (TNF-308GG, n = 225). The levels of IL-6 showed no statistical difference. The oxygenation index, serum creatinine level, 24-hour blood loss, intubation time, and length of ICU stay showed no statistical difference. Conclusions: TNF G-308A polymorphism may be associated with excess TNF-α secretion in this study and may not be associated with excess IL-6 secretion and postoperative morbidity after CPB.

Original languageEnglish
Pages (from-to)646-650
Number of pages5
JournalJournal of Cardiothoracic and Vascular Anesthesia
Volume23
Issue number5
DOIs
Publication statusPublished - 2009 Oct

Keywords

  • cardiopulmonary bypass
  • cytokine
  • morbidity
  • polymorphism
  • tumor necrosis factor α

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Anesthesiology and Pain Medicine

Fingerprint Dive into the research topics of 'Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population'. Together they form a unique fingerprint.

  • Cite this