Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population

Seung-Zhoo Yoon, In Jin Jang, Yoon Ji Choi, Mae Hwa Kang, Hae Ja Lim, Young Jin Lim, Hye Won Lee, Seong Ho Chang, Suk Min Yoon

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Abstract

Objectives: The G-308A polymorphism of the tumor necrosis factor α (TNF-α) gene has been suggested to be linked to high TNF promoter activity in in vitro studies. However, there have been some controversies in in vivo studies. This study investigated whether A allele at TNF-308 site is associated with (1) the changes in plasma cytokine levels during and after cardiopulmonary bypass (CPB) and (2) an increased incidence of pulmonary morbidity after CPB. Design: Prospective and observational investigation. Setting: A university hospital, single institution. Participants: Patients scheduled for cardiac surgery with CPB. Intervention: TNF genotype was determined by the real-time polymerase chain reaction method. IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay at the following time points: T1, before initiation of CPB; T2, 30 minutes of CPB; T3, 30 minutes after CPB; T4, 2 hours after CPB; and T5, 24 hours after CPB. The oxygen index, serum creatinine level, 24-hour blood loss, intubation time, and length of intensive care unit (ICU) stay were examined. Measurements and Main Results: The levels of TNF-α in group A (TNF-308GA/AA, n = 25) were higher at T3, T4, and T5 than group G (TNF-308GG, n = 225). The levels of IL-6 showed no statistical difference. The oxygenation index, serum creatinine level, 24-hour blood loss, intubation time, and length of ICU stay showed no statistical difference. Conclusions: TNF G-308A polymorphism may be associated with excess TNF-α secretion in this study and may not be associated with excess IL-6 secretion and postoperative morbidity after CPB.

Original languageEnglish
Pages (from-to)646-650
Number of pages5
JournalJournal of Cardiothoracic and Vascular Anesthesia
Volume23
Issue number5
DOIs
Publication statusPublished - 2009 Oct 1

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Keywords

  • cardiopulmonary bypass
  • cytokine
  • morbidity
  • polymorphism
  • tumor necrosis factor α

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Anesthesiology and Pain Medicine

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