Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population

Seung-Zhoo Yoon, In Jin Jang, Yoon Ji Choi, Mae Hwa Kang, Hae Ja Lim, Young Jin Lim, Hye Won Lee, Seong Ho Chang, Suk Min Yoon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives: The G-308A polymorphism of the tumor necrosis factor α (TNF-α) gene has been suggested to be linked to high TNF promoter activity in in vitro studies. However, there have been some controversies in in vivo studies. This study investigated whether A allele at TNF-308 site is associated with (1) the changes in plasma cytokine levels during and after cardiopulmonary bypass (CPB) and (2) an increased incidence of pulmonary morbidity after CPB. Design: Prospective and observational investigation. Setting: A university hospital, single institution. Participants: Patients scheduled for cardiac surgery with CPB. Intervention: TNF genotype was determined by the real-time polymerase chain reaction method. IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay at the following time points: T1, before initiation of CPB; T2, 30 minutes of CPB; T3, 30 minutes after CPB; T4, 2 hours after CPB; and T5, 24 hours after CPB. The oxygen index, serum creatinine level, 24-hour blood loss, intubation time, and length of intensive care unit (ICU) stay were examined. Measurements and Main Results: The levels of TNF-α in group A (TNF-308GA/AA, n = 25) were higher at T3, T4, and T5 than group G (TNF-308GG, n = 225). The levels of IL-6 showed no statistical difference. The oxygenation index, serum creatinine level, 24-hour blood loss, intubation time, and length of ICU stay showed no statistical difference. Conclusions: TNF G-308A polymorphism may be associated with excess TNF-α secretion in this study and may not be associated with excess IL-6 secretion and postoperative morbidity after CPB.

Original languageEnglish
Pages (from-to)646-650
Number of pages5
JournalJournal of Cardiothoracic and Vascular Anesthesia
Volume23
Issue number5
DOIs
Publication statusPublished - 2009 Oct 1

Fingerprint

Cardiopulmonary Bypass
Thoracic Surgery
Tumor Necrosis Factor-alpha
Cytokines
Population
Interleukin-6
Intubation
Intensive Care Units
Creatinine
Morbidity
Serum
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
Alleles
Genotype
Oxygen
Lung
Incidence
Genes

Keywords

  • cardiopulmonary bypass
  • cytokine
  • morbidity
  • polymorphism
  • tumor necrosis factor α

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Anesthesiology and Pain Medicine

Cite this

Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population. / Yoon, Seung-Zhoo; Jang, In Jin; Choi, Yoon Ji; Kang, Mae Hwa; Lim, Hae Ja; Lim, Young Jin; Lee, Hye Won; Chang, Seong Ho; Yoon, Suk Min.

In: Journal of Cardiothoracic and Vascular Anesthesia, Vol. 23, No. 5, 01.10.2009, p. 646-650.

Research output: Contribution to journalArticle

@article{57436f48fdaa4dddb2f6561cb4dc8fd5,
title = "Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population",
abstract = "Objectives: The G-308A polymorphism of the tumor necrosis factor α (TNF-α) gene has been suggested to be linked to high TNF promoter activity in in vitro studies. However, there have been some controversies in in vivo studies. This study investigated whether A allele at TNF-308 site is associated with (1) the changes in plasma cytokine levels during and after cardiopulmonary bypass (CPB) and (2) an increased incidence of pulmonary morbidity after CPB. Design: Prospective and observational investigation. Setting: A university hospital, single institution. Participants: Patients scheduled for cardiac surgery with CPB. Intervention: TNF genotype was determined by the real-time polymerase chain reaction method. IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay at the following time points: T1, before initiation of CPB; T2, 30 minutes of CPB; T3, 30 minutes after CPB; T4, 2 hours after CPB; and T5, 24 hours after CPB. The oxygen index, serum creatinine level, 24-hour blood loss, intubation time, and length of intensive care unit (ICU) stay were examined. Measurements and Main Results: The levels of TNF-α in group A (TNF-308GA/AA, n = 25) were higher at T3, T4, and T5 than group G (TNF-308GG, n = 225). The levels of IL-6 showed no statistical difference. The oxygenation index, serum creatinine level, 24-hour blood loss, intubation time, and length of ICU stay showed no statistical difference. Conclusions: TNF G-308A polymorphism may be associated with excess TNF-α secretion in this study and may not be associated with excess IL-6 secretion and postoperative morbidity after CPB.",
keywords = "cardiopulmonary bypass, cytokine, morbidity, polymorphism, tumor necrosis factor α",
author = "Seung-Zhoo Yoon and Jang, {In Jin} and Choi, {Yoon Ji} and Kang, {Mae Hwa} and Lim, {Hae Ja} and Lim, {Young Jin} and Lee, {Hye Won} and Chang, {Seong Ho} and Yoon, {Suk Min}",
year = "2009",
month = "10",
day = "1",
doi = "10.1053/j.jvca.2009.03.004",
language = "English",
volume = "23",
pages = "646--650",
journal = "Journal of Cardiothoracic and Vascular Anesthesia",
issn = "1053-0770",
publisher = "W.B. Saunders Ltd",
number = "5",

}

TY - JOUR

T1 - Association Between Tumor Necrosis Factor α 308G/A Polymorphism and Increased Proinflammatory Cytokine Release After Cardiac Surgery With Cardiopulmonary Bypass in the Korean Population

AU - Yoon, Seung-Zhoo

AU - Jang, In Jin

AU - Choi, Yoon Ji

AU - Kang, Mae Hwa

AU - Lim, Hae Ja

AU - Lim, Young Jin

AU - Lee, Hye Won

AU - Chang, Seong Ho

AU - Yoon, Suk Min

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Objectives: The G-308A polymorphism of the tumor necrosis factor α (TNF-α) gene has been suggested to be linked to high TNF promoter activity in in vitro studies. However, there have been some controversies in in vivo studies. This study investigated whether A allele at TNF-308 site is associated with (1) the changes in plasma cytokine levels during and after cardiopulmonary bypass (CPB) and (2) an increased incidence of pulmonary morbidity after CPB. Design: Prospective and observational investigation. Setting: A university hospital, single institution. Participants: Patients scheduled for cardiac surgery with CPB. Intervention: TNF genotype was determined by the real-time polymerase chain reaction method. IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay at the following time points: T1, before initiation of CPB; T2, 30 minutes of CPB; T3, 30 minutes after CPB; T4, 2 hours after CPB; and T5, 24 hours after CPB. The oxygen index, serum creatinine level, 24-hour blood loss, intubation time, and length of intensive care unit (ICU) stay were examined. Measurements and Main Results: The levels of TNF-α in group A (TNF-308GA/AA, n = 25) were higher at T3, T4, and T5 than group G (TNF-308GG, n = 225). The levels of IL-6 showed no statistical difference. The oxygenation index, serum creatinine level, 24-hour blood loss, intubation time, and length of ICU stay showed no statistical difference. Conclusions: TNF G-308A polymorphism may be associated with excess TNF-α secretion in this study and may not be associated with excess IL-6 secretion and postoperative morbidity after CPB.

AB - Objectives: The G-308A polymorphism of the tumor necrosis factor α (TNF-α) gene has been suggested to be linked to high TNF promoter activity in in vitro studies. However, there have been some controversies in in vivo studies. This study investigated whether A allele at TNF-308 site is associated with (1) the changes in plasma cytokine levels during and after cardiopulmonary bypass (CPB) and (2) an increased incidence of pulmonary morbidity after CPB. Design: Prospective and observational investigation. Setting: A university hospital, single institution. Participants: Patients scheduled for cardiac surgery with CPB. Intervention: TNF genotype was determined by the real-time polymerase chain reaction method. IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay at the following time points: T1, before initiation of CPB; T2, 30 minutes of CPB; T3, 30 minutes after CPB; T4, 2 hours after CPB; and T5, 24 hours after CPB. The oxygen index, serum creatinine level, 24-hour blood loss, intubation time, and length of intensive care unit (ICU) stay were examined. Measurements and Main Results: The levels of TNF-α in group A (TNF-308GA/AA, n = 25) were higher at T3, T4, and T5 than group G (TNF-308GG, n = 225). The levels of IL-6 showed no statistical difference. The oxygenation index, serum creatinine level, 24-hour blood loss, intubation time, and length of ICU stay showed no statistical difference. Conclusions: TNF G-308A polymorphism may be associated with excess TNF-α secretion in this study and may not be associated with excess IL-6 secretion and postoperative morbidity after CPB.

KW - cardiopulmonary bypass

KW - cytokine

KW - morbidity

KW - polymorphism

KW - tumor necrosis factor α

UR - http://www.scopus.com/inward/record.url?scp=70349305398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349305398&partnerID=8YFLogxK

U2 - 10.1053/j.jvca.2009.03.004

DO - 10.1053/j.jvca.2009.03.004

M3 - Article

C2 - 19467887

AN - SCOPUS:70349305398

VL - 23

SP - 646

EP - 650

JO - Journal of Cardiothoracic and Vascular Anesthesia

JF - Journal of Cardiothoracic and Vascular Anesthesia

SN - 1053-0770

IS - 5

ER -