Association of hepatitis B virus polymerase with promyelocytic leukemia nuclear bodies mediated by the S100 family protein p11

Juhyun Choi, Jin Sook Chang, Min Sup Song, Byung-Yoon Ahn, Young In Park, Dae Sik Lim, Ye Sun Han

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) polymerase (Pol) interacts with cellular chaperone proteins and thereby performs multiple functions necessary for viral replication. Yeast two-hybrid analysis was applied to identify additional cellular targets required for HBV Pol function. HBV Pol interacted with S100A10 (p11), a Ca2+-modulated protein previously shown to bind to annexin II. The interaction between HBV Pol and p11 was confirmed by co-immunoprecipitation of the two proteins synthesized either in vitro or in transfected cells and by inhibition of the DNA polymerase activity of HBV Pol by p11. Immunofluorescence analysis of transfected human cell lines revealed that, although most HBV Pol and p11 was restricted to the cytoplasm, a small proportion of each protein colocalized as nuclear speckles; HBV Pol was not detected in the nucleus in the absence of p11. The HBV Pol-p11 nuclear speckles coincided with nuclear bodies containing the promyelocytic leukemia protein PML. Furthermore, the association of HBV Pol-p11 with PML was increased by exposure of cells to EGTA and inhibited by valinomycin. These results suggest a role for p11 in modulation of HBV Pol function and implicate PML nuclear bodies and intracellular Ca2+ in viral replication.

Original languageEnglish
Pages (from-to)1049-1056
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume305
Issue number4
DOIs
Publication statusPublished - 2003 Jun 13

Fingerprint

Viruses
Hepatitis B virus
Leukemia
Speckle
Proteins
Annexin A2
S100 calcium binding protein A10
Valinomycin
Egtazic Acid
DNA-Directed DNA Polymerase
Immunoprecipitation
Yeast
Fluorescent Antibody Technique
Cytoplasm
Yeasts
Cells
Modulation
Cell Line

Keywords

  • Annexin II-binding protein
  • Hepatitis B virus polymerase
  • p11
  • PML nuclear bodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Association of hepatitis B virus polymerase with promyelocytic leukemia nuclear bodies mediated by the S100 family protein p11. / Choi, Juhyun; Chang, Jin Sook; Song, Min Sup; Ahn, Byung-Yoon; Park, Young In; Lim, Dae Sik; Han, Ye Sun.

In: Biochemical and Biophysical Research Communications, Vol. 305, No. 4, 13.06.2003, p. 1049-1056.

Research output: Contribution to journalArticle

Choi, Juhyun ; Chang, Jin Sook ; Song, Min Sup ; Ahn, Byung-Yoon ; Park, Young In ; Lim, Dae Sik ; Han, Ye Sun. / Association of hepatitis B virus polymerase with promyelocytic leukemia nuclear bodies mediated by the S100 family protein p11. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 305, No. 4. pp. 1049-1056.
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