Abstract
Pig breeding is aimed at improving lean meat production ability as well as meat quality, and muscle fibre characteristics may be important for enhancing these traits. Therefore, new molecular markers have been demanded for selecting lean meat production ability and meat quality in live animals. Myogenin belongs to the MyoD gene family, and is a candidate gene responsible for muscle fibre characteristics. We identified a new single nucleotide polymorphism (SNP) site in the 5′ upstream region of the myogenin gene (nucleotides C and T). A total of 252 pigs of three breeds were genotyped by polymerase chain reaction-restriction fragment length polymorphism using BspCNI. Additionally, they were genotyped for the previously detected MspI site in the 3′-flanking region (alleles A and B). The CCBB diplotype had the highest frequency over breeds, followed by TCBB and CCAB. The other diplotypes were not found in studied pigs. Association analysis performed for the markers found that the TCBB diplotype has desirable effects on the total number of fibres (p < 0.002), fibre cross-sectional area (p < 0.06), and loin eye area (p < 0.001) than the other diplotypes. Moreover, the diplotype had the highest muscle pH value (p < 0.07) and all meat quality traits were near the upper limit of the normal range as a reddish pink, firm and non-exudative (RFN) pork. Therefore, we suggest that selection for the myogenin diplotypes could improve total muscle fibre number, size and lean meat production ability with good meat quality.
| Original language | English |
|---|---|
| Pages (from-to) | 134-141 |
| Number of pages | 8 |
| Journal | Journal of Animal Breeding and Genetics |
| Volume | 126 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Apr 1 |
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Keywords
- Lean meat production
- Meat quality
- Muscle fibre number
- Myogenin
- Pig
ASJC Scopus subject areas
- Animal Science and Zoology
- Food Animals
Cite this
Associations of the variation in the porcine myogenin gene with muscle fibre characteristics, lean meat production and meat quality traits. / Kim, J. M.; Choi, B. D.; Kim, Byoung-Chul; Park, Sung Soo; Hong, Ki Chang.
In: Journal of Animal Breeding and Genetics, Vol. 126, No. 2, 01.04.2009, p. 134-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Associations of the variation in the porcine myogenin gene with muscle fibre characteristics, lean meat production and meat quality traits
AU - Kim, J. M.
AU - Choi, B. D.
AU - Kim, Byoung-Chul
AU - Park, Sung Soo
AU - Hong, Ki Chang
PY - 2009/4/1
Y1 - 2009/4/1
N2 - Pig breeding is aimed at improving lean meat production ability as well as meat quality, and muscle fibre characteristics may be important for enhancing these traits. Therefore, new molecular markers have been demanded for selecting lean meat production ability and meat quality in live animals. Myogenin belongs to the MyoD gene family, and is a candidate gene responsible for muscle fibre characteristics. We identified a new single nucleotide polymorphism (SNP) site in the 5′ upstream region of the myogenin gene (nucleotides C and T). A total of 252 pigs of three breeds were genotyped by polymerase chain reaction-restriction fragment length polymorphism using BspCNI. Additionally, they were genotyped for the previously detected MspI site in the 3′-flanking region (alleles A and B). The CCBB diplotype had the highest frequency over breeds, followed by TCBB and CCAB. The other diplotypes were not found in studied pigs. Association analysis performed for the markers found that the TCBB diplotype has desirable effects on the total number of fibres (p < 0.002), fibre cross-sectional area (p < 0.06), and loin eye area (p < 0.001) than the other diplotypes. Moreover, the diplotype had the highest muscle pH value (p < 0.07) and all meat quality traits were near the upper limit of the normal range as a reddish pink, firm and non-exudative (RFN) pork. Therefore, we suggest that selection for the myogenin diplotypes could improve total muscle fibre number, size and lean meat production ability with good meat quality.
AB - Pig breeding is aimed at improving lean meat production ability as well as meat quality, and muscle fibre characteristics may be important for enhancing these traits. Therefore, new molecular markers have been demanded for selecting lean meat production ability and meat quality in live animals. Myogenin belongs to the MyoD gene family, and is a candidate gene responsible for muscle fibre characteristics. We identified a new single nucleotide polymorphism (SNP) site in the 5′ upstream region of the myogenin gene (nucleotides C and T). A total of 252 pigs of three breeds were genotyped by polymerase chain reaction-restriction fragment length polymorphism using BspCNI. Additionally, they were genotyped for the previously detected MspI site in the 3′-flanking region (alleles A and B). The CCBB diplotype had the highest frequency over breeds, followed by TCBB and CCAB. The other diplotypes were not found in studied pigs. Association analysis performed for the markers found that the TCBB diplotype has desirable effects on the total number of fibres (p < 0.002), fibre cross-sectional area (p < 0.06), and loin eye area (p < 0.001) than the other diplotypes. Moreover, the diplotype had the highest muscle pH value (p < 0.07) and all meat quality traits were near the upper limit of the normal range as a reddish pink, firm and non-exudative (RFN) pork. Therefore, we suggest that selection for the myogenin diplotypes could improve total muscle fibre number, size and lean meat production ability with good meat quality.
KW - Lean meat production
KW - Meat quality
KW - Muscle fibre number
KW - Myogenin
KW - Pig
UR - http://www.scopus.com/inward/record.url?scp=62549091396&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62549091396&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0388.2008.00724.x
DO - 10.1111/j.1439-0388.2008.00724.x
M3 - Article
C2 - 19320770
AN - SCOPUS:62549091396
VL - 126
SP - 134
EP - 141
JO - Journal of Animal Breeding and Genetics
JF - Journal of Animal Breeding and Genetics
SN - 0931-2668
IS - 2
ER -