Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression

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6 Citations (Scopus)

Abstract

Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalGut and Liver
Volume10
Issue number3
DOIs
Publication statusPublished - 2016 Jan 1

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Stomach Neoplasms
Cell Cycle
Cell Line
Extracellular Signal-Regulated MAP Kinases
Stomach
Cell Cycle Resting Phase
astaxanthine
G1 Phase
Carotenoids
S Phase
Immunoblotting
Cell Survival
Adenocarcinoma
Anti-Inflammatory Agents
Cell Count
Antioxidants
Phosphorylation
Carcinoma
Neoplasms

Keywords

  • Astaxanthin
  • Extracellular signal-regulated kinase
  • Human gastric adenocarcinoma
  • P27
  • Proliferation

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

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title = "Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression",
abstract = "Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.",
keywords = "Astaxanthin, Extracellular signal-regulated kinase, Human gastric adenocarcinoma, P27, Proliferation",
author = "Kim, {Jung Ha} and Park, {Jong Jae} and Beomjae Lee and Joo, {Moon Kyung} and Hoon-Jai Chun and Lee, {Sang Woo} and Young-Tae Bak",
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pages = "369--374",
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issn = "1976-2283",
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T1 - Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression

AU - Kim, Jung Ha

AU - Park, Jong Jae

AU - Lee, Beomjae

AU - Joo, Moon Kyung

AU - Chun, Hoon-Jai

AU - Lee, Sang Woo

AU - Bak, Young-Tae

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.

AB - Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.

KW - Astaxanthin

KW - Extracellular signal-regulated kinase

KW - Human gastric adenocarcinoma

KW - P27

KW - Proliferation

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