Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression

Jung Ha Kim; Jong Jae Park; Beom Jae Lee; Moon Kyung Joo; Hoon Jai Chun; Sang Woo Lee; Young Tae Bak

doi:10.5009/gnl15208

Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression

Jung Ha Kim, Jong Jae Park, Beom Jae Lee, Moon Kyung Joo, Hoon Jai Chun, Sang Woo Lee, Young Tae Bak

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27^kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27^kip-1.

Original language	English
Pages (from-to)	369-374
Number of pages	6
Journal	Gut and liver
Volume	10
Issue number	3
DOIs	https://doi.org/10.5009/gnl15208
Publication status	Published - 2016
Externally published	Yes

Keywords

Astaxanthin
Extracellular signal-regulated kinase
Human gastric adenocarcinoma
P27
Proliferation

ASJC Scopus subject areas

Hepatology
Gastroenterology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.5009/gnl15208

Cite this

@article{814b098b291443b2939f6278884084a7,

title = "Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression",

abstract = "Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.",

keywords = "Astaxanthin, Extracellular signal-regulated kinase, Human gastric adenocarcinoma, P27, Proliferation",

author = "Kim, {Jung Ha} and Park, {Jong Jae} and Lee, {Beom Jae} and Joo, {Moon Kyung} and Chun, {Hoon Jai} and Lee, {Sang Woo} and Bak, {Young Tae}",

note = "Publisher Copyright: {\textcopyright} This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License.",

year = "2016",

doi = "10.5009/gnl15208",

language = "English",

volume = "10",

pages = "369--374",

journal = "Gut and Liver",

issn = "1976-2283",

publisher = "Joe Bok Chung",

number = "3",

}

TY - JOUR

T1 - Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression

AU - Kim, Jung Ha

AU - Park, Jong Jae

AU - Lee, Beom Jae

AU - Joo, Moon Kyung

AU - Chun, Hoon Jai

AU - Lee, Sang Woo

AU - Bak, Young Tae

N1 - Publisher Copyright: © This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License.

PY - 2016

Y1 - 2016

N2 - Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.

AB - Background/Aims: Astaxanthin is a carotenoid pigment that has antioxidant, antitumoral, and anti-inflammatory properties. In this in vitro study, we investigated the mechanism of anticancer effects of astaxanthin in gastric carcinoma cell lines. Methods: The human gastric adenocarcinoma cell lines AGS, KATO-III, MKN-45, and SNU-1 were treated with various concentrations of astaxanthin. A cell viability test, cell cycle analysis, and immunoblotting were performed. Results: The viability of each cancer cell line was suppressed by astaxanthin in a dose-dependent manner with significantly decreased proliferation in KATO-III and SNU-1 cells. Astaxanthin increased the number of cells in the G0/G1 phase but reduced the proportion of S phase KATO-III and SNU-1 cells. Phosphorylated extracellular signal-regulated kinase (ERK) was decreased in an inverse dose-dependent correlation with astaxanthin concentration, and the expression of p27kip-1 increased the KATO-III and SNU-1 cell lines in an astaxanthin dose-dependent manner. Conclusions: Astaxanthin inhibits proliferation by interrupting cell cycle progression in KATO-III and SNU-1 gastric cancer cells. This may be caused by the inhibition of the phosphorylation of ERK and the enhanced expression of p27kip-1.

KW - Astaxanthin

KW - Extracellular signal-regulated kinase

KW - Human gastric adenocarcinoma

KW - P27

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=84969165003&partnerID=8YFLogxK

U2 - 10.5009/gnl15208

DO - 10.5009/gnl15208

M3 - Article

C2 - 26470770

AN - SCOPUS:84969165003

SN - 1976-2283

VL - 10

SP - 369

EP - 374

JO - Gut and Liver

JF - Gut and Liver

IS - 3

ER -

Astaxanthin inhibits proliferation of human gastric cancer cell lines by interrupting cell cycle progression

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this