Abstract
Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT-PCR to determine the levels of stress-response gene transcripts in wild-type and atmyb7 plants. We showed that ArabidopsisAtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high-salt stress conditions, atmyb7 plants showed a lower germination rate than did wild-type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis.
Original language | English |
---|---|
Pages (from-to) | 559-571 |
Number of pages | 13 |
Journal | Plant, Cell and Environment |
Volume | 38 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2015 Mar 1 |
Fingerprint
Keywords
- Arabidopsis thaliana
- MYB transcription factor
ASJC Scopus subject areas
- Plant Science
- Physiology
Cite this
AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA-induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5. / Kim, Jun Hyeok; Hyun, Woo Young; Nguyen, Hoai Nguyen; Jeong, Chan Young; Xiong, Liming; Hong, Suk Whan; Lee, Hojoung.
In: Plant, Cell and Environment, Vol. 38, No. 3, 01.03.2015, p. 559-571.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA-induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5
AU - Kim, Jun Hyeok
AU - Hyun, Woo Young
AU - Nguyen, Hoai Nguyen
AU - Jeong, Chan Young
AU - Xiong, Liming
AU - Hong, Suk Whan
AU - Lee, Hojoung
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT-PCR to determine the levels of stress-response gene transcripts in wild-type and atmyb7 plants. We showed that ArabidopsisAtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high-salt stress conditions, atmyb7 plants showed a lower germination rate than did wild-type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis.
AB - Various Myb proteins have been shown to play crucial roles in plants, including primary and secondary metabolism, determination of cell fate and identity, regulation of development and involvement in responses to biotic and abiotic stresses. The 126 R2R3 Myb proteins (with two Myb repeats) have been found in Arabidopsis; however, the functions of most of these proteins remain to be fully elucidated. In the present study, we characterized the function of AtMyb7 using molecular biological and genetic analyses. We used qRT-PCR to determine the levels of stress-response gene transcripts in wild-type and atmyb7 plants. We showed that ArabidopsisAtMyb7 plays a critical role in seed germination. Under abscisic acid (ABA) and high-salt stress conditions, atmyb7 plants showed a lower germination rate than did wild-type plants. Furthermore, AtMyb7 promoter:GUS seeds exhibited different expression patterns in response to variations in the seed imbibition period. AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in ABA signalling and serves as a crucial regulator of germination inhibition in Arabidopsis.
KW - Arabidopsis thaliana
KW - MYB transcription factor
UR - http://www.scopus.com/inward/record.url?scp=84922595633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922595633&partnerID=8YFLogxK
U2 - 10.1111/pce.12415
DO - 10.1111/pce.12415
M3 - Article
C2 - 25053018
AN - SCOPUS:84922595633
VL - 38
SP - 559
EP - 571
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 3
ER -