ATP-dependent RecG helicase is required for the transcriptional regulator OxyR function in Pseudomonas species

Jinki Yeom, Yunho Lee, Woojun Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The oxyR gene appears to reside in an operon with the recG helicase gene in many bacteria, including pathogenic Pseudomonas aeruginosa and Pseudomonas putida. Analysis of P. putida transcriptomes shows that many OxyR-controlled genes are regulated by the ATP-dependent RecG helicase and that RecG alone modulates the expression of many genes. We found that purified RecG binds to the promoters of many OxyR-controlled genes and that expression of these genes was not induced under conditions of oxidative stress in recG mutants of P. aeruginosa, P. putida, and Escherichia coli. In vitro data revealed that promoters containing palindromic sequences are essential for RecG binding and that single-strand binding proteins and ATP are also needed for RecG to promote transcription, whereas a magnesium ion has the opposite effect. The OxyR tetramer preferentially binds to promoters after RecG has generated linear DNA in the presence of ATP; otherwise, the OxyR dimer has higher affinity. This study provides new insights into the mechanism of bacterial transcription by demonstrating that RecG might be required for the induction of the OxyR regulon by unwinding palindromic DNA for transcription. This work describes a novel bacterial transcriptional function by RecG helicase with OxyR and may provide new targets for controlling Pseudomonas species pathogen.

Original languageEnglish
Pages (from-to)24492-24504
Number of pages13
JournalJournal of Biological Chemistry
Volume287
Issue number29
DOIs
Publication statusPublished - 2012 Jul 13

Fingerprint

Pseudomonas
Pseudomonas putida
Genes
Adenosine Triphosphate
Transcription
Pseudomonas aeruginosa
Gene Expression
Regulon
DNA
Operon
Transcriptome
Magnesium
Carrier Proteins
Oxidative Stress
Oxidative stress
Pathogens
Ions
Escherichia coli
Bacteria
Dimers

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

ATP-dependent RecG helicase is required for the transcriptional regulator OxyR function in Pseudomonas species. / Yeom, Jinki; Lee, Yunho; Park, Woojun.

In: Journal of Biological Chemistry, Vol. 287, No. 29, 13.07.2012, p. 24492-24504.

Research output: Contribution to journalArticle

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