Attenuation of coxsackievirus B3 by VP2 mutation and its application as a vaccine against virus-induced myocarditis and pancreatitis

Jung Hyun Park, Dae Sun Kim, Young Joo Cho, Yeon Jung Kim, Soo Young Jeong, Seung Min Lee, Seong Joo Cho, Cheol Won Yun, Inho Jo, Jae Hwan Nam

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Coxsackievirus B3 (CVB3) is a common agent of viral myocarditis, a major cause of sudden cardiac death, and ultimately dilated cardiomyopathy. However, there is no vaccine in clinical use. In this study, we identified the conserved amino acid sequences in the C-terminal region of the VP2 of the coxsackievirus B group and some echoviruses. The mutant virus, YYFF, with phenylalanines substituted for two tyrosines in these conserved sequences was highly attenuated in vivo and could induce a high neutralizing antibody titer and a cytotoxic T-lymphocyte response against CVB3. Thereby, mutant-virus-immunized mice showed a 100% survival rate and protection against inflammation of the heart and pancreas after lethal dose challenge. Thus, this mutant virus is a good candidate for an attenuated CVB3 vaccine.

Original languageEnglish
Pages (from-to)1974-1983
Number of pages10
JournalVaccine
Volume27
Issue number13
DOIs
Publication statusPublished - 2009 Mar 18

Keywords

  • Attenuated vaccine
  • Coxsackievirus
  • VP2

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Attenuation of coxsackievirus B3 by VP2 mutation and its application as a vaccine against virus-induced myocarditis and pancreatitis'. Together they form a unique fingerprint.

  • Cite this

    Park, J. H., Kim, D. S., Cho, Y. J., Kim, Y. J., Jeong, S. Y., Lee, S. M., Cho, S. J., Yun, C. W., Jo, I., & Nam, J. H. (2009). Attenuation of coxsackievirus B3 by VP2 mutation and its application as a vaccine against virus-induced myocarditis and pancreatitis. Vaccine, 27(13), 1974-1983. https://doi.org/10.1016/j.vaccine.2009.01.008