Attenuation of inflammation and cartilage degradation by sulfasalazine-containing hyaluronic acid on osteoarthritis rat model

Sung Eun Kim, Jae Yong Lee, Kyu Sik Shim, Sunghee Lee, Kyoengwoo Min, Ji Hoon Bae, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study was to investigate the effects of a sulfasalazine-containing hyaluronic acid (SASP/HA) systems on in vitro anti-inflammation and the alleviation of cartilage degradation in both lipopolysaccharide (LPS)-stimulated synoviocytes and a rat model of monosodium iodoacetate (MIA)-induced osteoarthritis (OA). The SASP/HA resulted in long-term release of SASP from the SASP/HA for up to 60 days in a sustained manner. In vitro studies performed using real-time polymerase chain reaction (PCR) assay revealed that the SASP/HA was able to effectively and dose-dependently inhibit the mRNA expression levels of pro-inflammatory cytokines such as matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in LPS-stimulated synoviocytes. In vivo studies showed that intra articular injection of SASP/HA greatly reduced the MIA-stimulated mRNA expression of MMP-3, COX-2, IL-6, and TNF-α in blood. Furthermore, these significant anti-inflammatory effects of SASP/HA contributed markedly to the alleviation of progression of MIA-induced OA and cartilage degradation, as demonstrated by X-ray, micro-computed tomography (micro-CT), gross findings, and histological evaluations. Therefore, our findings indicated that the long-term and sustained delivery of SASP using HA can play a therapeutic role in alleviating inflammation as well as protecting against cartilage damage in OA.

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume114
DOIs
Publication statusPublished - 2018 Jul 15

Keywords

  • Hyaluronic acid
  • Osteoarthritis
  • Sulfasalazine

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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