Avian SERPINB11 gene: A marker for ovarian endometrioid cancer in chickens

Whasun Lim, Ji Hye Kim, Suzie E. Ahn, Wooyoung Jeong, Jinyoung Kim, Fuller W. Bazer, Jae Yong Han, Gwonhwa Song

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

As serine and cysteine proteinase inhibitors, serpins, such as SERPINB5, cause ovarian, colorectal and pancreatic adenocarcinomas. We identified SERPINB11 as a novel estrogen-induced gene in chickens during oviduct development. The chicken is a unique animal model for research on human ovarian cancer, because it spontaneously develops epithelial cell-derived ovarian cancer as in women. Therefore, this study investigated the expression pattern, CpG methylation status, and miRNA regulation of the SERPINB11 gene in normal and cancerous ovaries from chickens. Our results indicate that SERPINB11 is most abundant in the glandular epithelium of endometrioid adenocarcinoma of cancerous, but not normal, ovaries of hens. In addition, bisulfite sequencing revealed that about 30% of 2110 CpG sites are methylated in ovarian cancer cells, whereas 2110 CpG sites are demethylated in normal ovarian cells. Next, we determined whether miR-1582 influences SERPINB11 expression via its 3'UTR and found that it does not directly target the 3'UTR of SERPINB11 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences SERPINB11 expression in the chicken ovary. On the other hand, in human ovarian cancer cells such as OVCAR-3, SKOV-3 and PA-1 cells, immunoreactive SERPINB11 protein was predominant in the cytoplasm and had a similar expression pattern to that in chicken ovarian cancer cells. Collectively, these results suggest that SERPINB11 is a biomarker for chicken ovarian endometrioid carcinoma that could be used for diagnosis and monitoring effects of therapies for the disease in women.

Original languageEnglish
Pages (from-to)150-159
Number of pages10
JournalExperimental Biology and Medicine
Volume237
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1
Externally publishedYes

Fingerprint

Ovarian Neoplasms
Chickens
Genes
Cells
3' Untranslated Regions
Serpins
Endometrioid Carcinoma
Cysteine Proteinase Inhibitors
Ovary
Serine Proteinase Inhibitors
Methylation
Biomarkers
MicroRNAs
Estrogens
Animals
Messenger RNA
Oviducts
Monitoring
Cytoplasm
Proteins

Keywords

  • Cancer
  • Chicken
  • Ovary
  • SERPINB11

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Avian SERPINB11 gene : A marker for ovarian endometrioid cancer in chickens. / Lim, Whasun; Kim, Ji Hye; Ahn, Suzie E.; Jeong, Wooyoung; Kim, Jinyoung; Bazer, Fuller W.; Han, Jae Yong; Song, Gwonhwa.

In: Experimental Biology and Medicine, Vol. 237, No. 2, 01.02.2012, p. 150-159.

Research output: Contribution to journalArticle

Lim, W, Kim, JH, Ahn, SE, Jeong, W, Kim, J, Bazer, FW, Han, JY & Song, G 2012, 'Avian SERPINB11 gene: A marker for ovarian endometrioid cancer in chickens', Experimental Biology and Medicine, vol. 237, no. 2, pp. 150-159. https://doi.org/10.1258/ebm.2011.011250
Lim, Whasun ; Kim, Ji Hye ; Ahn, Suzie E. ; Jeong, Wooyoung ; Kim, Jinyoung ; Bazer, Fuller W. ; Han, Jae Yong ; Song, Gwonhwa. / Avian SERPINB11 gene : A marker for ovarian endometrioid cancer in chickens. In: Experimental Biology and Medicine. 2012 ; Vol. 237, No. 2. pp. 150-159.
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