Abstract
As serine and cysteine proteinase inhibitors, serpins, such as SERPINB5, cause ovarian, colorectal and pancreatic adenocarcinomas. We identified SERPINB11 as a novel estrogen-induced gene in chickens during oviduct development. The chicken is a unique animal model for research on human ovarian cancer, because it spontaneously develops epithelial cell-derived ovarian cancer as in women. Therefore, this study investigated the expression pattern, CpG methylation status, and miRNA regulation of the SERPINB11 gene in normal and cancerous ovaries from chickens. Our results indicate that SERPINB11 is most abundant in the glandular epithelium of endometrioid adenocarcinoma of cancerous, but not normal, ovaries of hens. In addition, bisulfite sequencing revealed that about 30% of 2110 CpG sites are methylated in ovarian cancer cells, whereas 2110 CpG sites are demethylated in normal ovarian cells. Next, we determined whether miR-1582 influences SERPINB11 expression via its 3'UTR and found that it does not directly target the 3'UTR of SERPINB11 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences SERPINB11 expression in the chicken ovary. On the other hand, in human ovarian cancer cells such as OVCAR-3, SKOV-3 and PA-1 cells, immunoreactive SERPINB11 protein was predominant in the cytoplasm and had a similar expression pattern to that in chicken ovarian cancer cells. Collectively, these results suggest that SERPINB11 is a biomarker for chicken ovarian endometrioid carcinoma that could be used for diagnosis and monitoring effects of therapies for the disease in women.
| Original language | English |
|---|---|
| Pages (from-to) | 150-159 |
| Number of pages | 10 |
| Journal | Experimental Biology and Medicine |
| Volume | 237 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Feb 1 |
| Externally published | Yes |
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Keywords
- Cancer
- Chicken
- Ovary
- SERPINB11
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Avian SERPINB11 gene : A marker for ovarian endometrioid cancer in chickens. / Lim, Whasun; Kim, Ji Hye; Ahn, Suzie E.; Jeong, Wooyoung; Kim, Jinyoung; Bazer, Fuller W.; Han, Jae Yong; Song, Gwonhwa.
In: Experimental Biology and Medicine, Vol. 237, No. 2, 01.02.2012, p. 150-159.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Avian SERPINB11 gene
T2 - A marker for ovarian endometrioid cancer in chickens
AU - Lim, Whasun
AU - Kim, Ji Hye
AU - Ahn, Suzie E.
AU - Jeong, Wooyoung
AU - Kim, Jinyoung
AU - Bazer, Fuller W.
AU - Han, Jae Yong
AU - Song, Gwonhwa
PY - 2012/2/1
Y1 - 2012/2/1
N2 - As serine and cysteine proteinase inhibitors, serpins, such as SERPINB5, cause ovarian, colorectal and pancreatic adenocarcinomas. We identified SERPINB11 as a novel estrogen-induced gene in chickens during oviduct development. The chicken is a unique animal model for research on human ovarian cancer, because it spontaneously develops epithelial cell-derived ovarian cancer as in women. Therefore, this study investigated the expression pattern, CpG methylation status, and miRNA regulation of the SERPINB11 gene in normal and cancerous ovaries from chickens. Our results indicate that SERPINB11 is most abundant in the glandular epithelium of endometrioid adenocarcinoma of cancerous, but not normal, ovaries of hens. In addition, bisulfite sequencing revealed that about 30% of 2110 CpG sites are methylated in ovarian cancer cells, whereas 2110 CpG sites are demethylated in normal ovarian cells. Next, we determined whether miR-1582 influences SERPINB11 expression via its 3'UTR and found that it does not directly target the 3'UTR of SERPINB11 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences SERPINB11 expression in the chicken ovary. On the other hand, in human ovarian cancer cells such as OVCAR-3, SKOV-3 and PA-1 cells, immunoreactive SERPINB11 protein was predominant in the cytoplasm and had a similar expression pattern to that in chicken ovarian cancer cells. Collectively, these results suggest that SERPINB11 is a biomarker for chicken ovarian endometrioid carcinoma that could be used for diagnosis and monitoring effects of therapies for the disease in women.
AB - As serine and cysteine proteinase inhibitors, serpins, such as SERPINB5, cause ovarian, colorectal and pancreatic adenocarcinomas. We identified SERPINB11 as a novel estrogen-induced gene in chickens during oviduct development. The chicken is a unique animal model for research on human ovarian cancer, because it spontaneously develops epithelial cell-derived ovarian cancer as in women. Therefore, this study investigated the expression pattern, CpG methylation status, and miRNA regulation of the SERPINB11 gene in normal and cancerous ovaries from chickens. Our results indicate that SERPINB11 is most abundant in the glandular epithelium of endometrioid adenocarcinoma of cancerous, but not normal, ovaries of hens. In addition, bisulfite sequencing revealed that about 30% of 2110 CpG sites are methylated in ovarian cancer cells, whereas 2110 CpG sites are demethylated in normal ovarian cells. Next, we determined whether miR-1582 influences SERPINB11 expression via its 3'UTR and found that it does not directly target the 3'UTR of SERPINB11 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences SERPINB11 expression in the chicken ovary. On the other hand, in human ovarian cancer cells such as OVCAR-3, SKOV-3 and PA-1 cells, immunoreactive SERPINB11 protein was predominant in the cytoplasm and had a similar expression pattern to that in chicken ovarian cancer cells. Collectively, these results suggest that SERPINB11 is a biomarker for chicken ovarian endometrioid carcinoma that could be used for diagnosis and monitoring effects of therapies for the disease in women.
KW - Cancer
KW - Chicken
KW - Ovary
KW - SERPINB11
UR - http://www.scopus.com/inward/record.url?scp=84863116176&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863116176&partnerID=8YFLogxK
U2 - 10.1258/ebm.2011.011250
DO - 10.1258/ebm.2011.011250
M3 - Article
C2 - 22289513
AN - SCOPUS:84863116176
VL - 237
SP - 150
EP - 159
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 1535-3702
IS - 2
ER -