Avian serum response factor expression restricted primarily to muscle cell lineages is required for α-actin gene transcription

Jeffrey D. Croissant, Jae-Hong Kim, Gregor Eichele, Lisa Goering, John Lough, Ron Prywes, Robert J. Schwartz

Research output: Contribution to journalArticle

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Abstract

Serum response factor (SRF) gene expression in avian embryonic muscle lineages plays a central role in activating α-actin gene activity. In early stage HH 6 avian embryos, SRF mRNA expression showed strong localization to the neural groove, primitive streak, lateral plate mesoderm, and Hensen's node, while distinct SRF expression was seen later in the neural folds and the somites by HH stage 8. SRF transcripts appeared in the precardiac splanchnic mesoderm in stage HH 9 embryos and was detected at higher levels in the myocardium, somites, and lateral mesoderm of HH 11 embryos, SRF antibody staining demonstrated significant SRF protein accumulation in the myocardium of the developing heart and the myotomal portion of somites. During primary myogenesis in culture, SRF transcripts and nuclear SRF protein content increased about 40-fold, as primary myoblasts withdrew from the cell cycle, reaching their highest levels prior to the upregulation of the skeletal α-actin gene. A dominant-negative SRF mutant, SRFpm1, which inhibited DNA binding, but not dimerization of monomeric SRF subunits, blocked transcriptional activation of a skeletal α-actin promoter-luciferase reporter gene during myogenesis. Transcriptional blockade was reversed by co-transfections of a wild-type SRF expression vector, but was not rescued by the expression of other myogenic factors, such as MyoD and Mef-2C. Thus, SRF displayed an embryonic expression pattern restricted primarily to striated muscle cell lineages, in which increased mass of nuclear SRF was obligatory for α-actin gene transcription.

Original languageEnglish
Pages (from-to)250-264
Number of pages15
JournalDevelopmental Biology
Volume177
Issue number1
DOIs
Publication statusPublished - 1996 Jul 10
Externally publishedYes

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Serum Response Factor
Cell Lineage
Muscle Cells
Actins
Genes
Somites
Mesoderm
Embryonic Structures
Muscle Development
Neural Crest
Myocardium
Embryonic Organizers
Primitive Streak
Striated Muscle
Viscera
Myoblasts
Dimerization
Luciferases
Reporter Genes

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Avian serum response factor expression restricted primarily to muscle cell lineages is required for α-actin gene transcription. / Croissant, Jeffrey D.; Kim, Jae-Hong; Eichele, Gregor; Goering, Lisa; Lough, John; Prywes, Ron; Schwartz, Robert J.

In: Developmental Biology, Vol. 177, No. 1, 10.07.1996, p. 250-264.

Research output: Contribution to journalArticle

Croissant, Jeffrey D. ; Kim, Jae-Hong ; Eichele, Gregor ; Goering, Lisa ; Lough, John ; Prywes, Ron ; Schwartz, Robert J. / Avian serum response factor expression restricted primarily to muscle cell lineages is required for α-actin gene transcription. In: Developmental Biology. 1996 ; Vol. 177, No. 1. pp. 250-264.
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