Axin expression reduces staurosporine-induced mitochondria-mediated cell death in HeLa cells

Jee Hye Shin, Hyun wook Kim, Im Joo Rhyu, Ki-Joon Song, Sun-Ho Kee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cytoplasmic axin expression frequently produces punctuate structures in cells, but the nature of axin puncta has not been fully elucidated. In an effort to analyze cytoplasmic axin puncta, we established HeLa cells expressing axin in a doxycycline-inducible manner (HeLa-Axin). We observed that axin accumulated in an aggregate-like pattern in perinuclear areas and appeared to be associated with mitochondria, Golgi apparatus, and endoplasmic reticulum (ER), but not lysosomes. Further biochemical analysis suggested that some part of the cytoplasmic axin pool was associated with mitochondria. In addition, mitochondrial proteins [i.e., cytochrome oxidase IV (CoxIV) and cytochrome c] were slightly higher in HeLa-Axin cells than in HeLa-EV cells, suggesting altered mitochondrial degradation. HeLa-Axin cells were then treated with staurosporine (STS) to determine if the mitochondria-induced apoptosis pathway was altered. Compared to STS-treated control cells (HeLa-EV), HeLa-Axin cells had less STS-induced cytotoxicity and reduced caspase-3 activation and PARP cleavage. Given that mitochondria outer membrane potential was unchanged, HeLa-Axin cells might be relatively resistant to STS-mediated mitochondrial damage. Mitochondria associated with axin aggregates were resistant to detergent-mediated permeabilization. These results suggest that axin forms aggregate-like structures in association with mitochondria, which render mitochondria resistant to STS-induced membrane damage and cytotoxicity.

Original languageEnglish
Pages (from-to)2022-2033
Number of pages12
JournalExperimental Cell Research
Volume318
Issue number16
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Staurosporine
HeLa Cells
Mitochondria
Cell Death
Mitochondrial Degradation
Doxycycline
Mitochondrial Proteins
Golgi Apparatus
Electron Transport Complex IV
Cytochromes c
Lysosomes
Caspase 3
Endoplasmic Reticulum
Detergents
Membrane Potentials
Apoptosis
Membranes

Keywords

  • Axin
  • Cell death
  • Mitochondria
  • Staurosporine

ASJC Scopus subject areas

  • Cell Biology

Cite this

Axin expression reduces staurosporine-induced mitochondria-mediated cell death in HeLa cells. / Shin, Jee Hye; Kim, Hyun wook; Rhyu, Im Joo; Song, Ki-Joon; Kee, Sun-Ho.

In: Experimental Cell Research, Vol. 318, No. 16, 01.10.2012, p. 2022-2033.

Research output: Contribution to journalArticle

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