Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling

Mi Ri Park, Sangnam Oh, Seok Jun Son, Dong June Park, Sejong Oh, Sae Hun Kim, Do Youn Jeong, Nam Su Oh, Youngbok Lee, Minho Song, Younghoon Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we investigated potentially probiotic Bacillus licheniformis strains isolated from traditional Korean food sources for ability to enhance longevity using the nematode Caenorhabditis elegans as a simple in vivo animal model. We first investigated whether B. licheniformis strains were capable of modulating the lifespan of C. elegans. Among the tested strains, preconditioning with four B. licheniformis strains significantly enhanced the longevity of C. elegans. Unexpectedly, plate counting and transmission electron microscopy (TEM) results indicated that B. licheniformis strains were not more highly attached to the C. elegans intestine compared with Escherichia coli OP50 or Lactobacillus rhamnosus GG controls. In addition, qRT-PCR and an aging assay with mutant worms showed that the conditioning of B. licheniformis strain 141 directly influenced genes associated with serotonin signaling in nematodes, including tph-1 (tryptophan hydroxylase), bas-1 (serotonin- and dopamine-synthetic aromatic amino acid decarboxylase), mod-1 (serotonin-gated chloride channel), ser-1, and ser-7 (serotonin receptors) during C. elegans aging. Our findings suggest that B. licheniformis strain 141, which is isolated from traditional Korean foods, is a probiotic generally recognized as safe (GRAS) strain that enhances the lifespan of C. elegans via host serotonin signaling.

Original languageEnglish
Pages (from-to)10227-10233
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number47
DOIs
Publication statusPublished - 2015 Nov 6

Fingerprint

Bacillus licheniformis
Caenorhabditis elegans
Bacilli
serotonin
Serotonin
Food
Lactobacillus rhamnosus
Probiotics
Aromatic-L-Amino-Acid Decarboxylases
probiotics
Tryptophan Hydroxylase
Aging of materials
Chloride Channels
Nematoda
Transmission Electron Microscopy
chloride channels
Intestines
Dopamine
Escherichia coli
Animal Models

Keywords

  • antiaging
  • Bacillus licheniformis
  • Caenorhabditis elegans
  • Korean traditional foods
  • serotonin signaling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling. / Park, Mi Ri; Oh, Sangnam; Son, Seok Jun; Park, Dong June; Oh, Sejong; Kim, Sae Hun; Jeong, Do Youn; Oh, Nam Su; Lee, Youngbok; Song, Minho; Kim, Younghoon.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 47, 06.11.2015, p. 10227-10233.

Research output: Contribution to journalArticle

Park, Mi Ri ; Oh, Sangnam ; Son, Seok Jun ; Park, Dong June ; Oh, Sejong ; Kim, Sae Hun ; Jeong, Do Youn ; Oh, Nam Su ; Lee, Youngbok ; Song, Minho ; Kim, Younghoon. / Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 47. pp. 10227-10233.
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