Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling

Mi Ri Park; Sangnam Oh; Seok Jun Son; Dong June Park; Sejong Oh; Sae Hun Kim; Do Youn Jeong; Nam Su Oh; Youngbok Lee; Minho Song; Younghoon Kim

doi:10.1021/acs.jafc.5b03730

Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling

Mi Ri Park, Sangnam Oh, Seok Jun Son, Dong June Park, Sejong Oh, Sae Hun Kim, Do Youn Jeong, Nam Su Oh, Youngbok Lee, Minho Song, Younghoon Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

In this study, we investigated potentially probiotic Bacillus licheniformis strains isolated from traditional Korean food sources for ability to enhance longevity using the nematode Caenorhabditis elegans as a simple in vivo animal model. We first investigated whether B. licheniformis strains were capable of modulating the lifespan of C. elegans. Among the tested strains, preconditioning with four B. licheniformis strains significantly enhanced the longevity of C. elegans. Unexpectedly, plate counting and transmission electron microscopy (TEM) results indicated that B. licheniformis strains were not more highly attached to the C. elegans intestine compared with Escherichia coli OP50 or Lactobacillus rhamnosus GG controls. In addition, qRT-PCR and an aging assay with mutant worms showed that the conditioning of B. licheniformis strain 141 directly influenced genes associated with serotonin signaling in nematodes, including tph-1 (tryptophan hydroxylase), bas-1 (serotonin- and dopamine-synthetic aromatic amino acid decarboxylase), mod-1 (serotonin-gated chloride channel), ser-1, and ser-7 (serotonin receptors) during C. elegans aging. Our findings suggest that B. licheniformis strain 141, which is isolated from traditional Korean foods, is a probiotic generally recognized as safe (GRAS) strain that enhances the lifespan of C. elegans via host serotonin signaling.

Original language	English
Pages (from-to)	10227-10233
Number of pages	7
Journal	Journal of agricultural and food chemistry
Volume	63
Issue number	47
DOIs	https://doi.org/10.1021/acs.jafc.5b03730
Publication status	Published - 2015 Nov 6

Keywords

Bacillus licheniformis
Caenorhabditis elegans
Korean traditional foods
antiaging
serotonin signaling

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

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10.1021/acs.jafc.5b03730

Cite this

Park, M. R., Oh, S., Son, S. J., Park, D. J., Oh, S., Kim, S. H., Jeong, D. Y., Oh, N. S., Lee, Y., Song, M., & Kim, Y. (2015). Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling. Journal of agricultural and food chemistry, 63(47), 10227-10233. https://doi.org/10.1021/acs.jafc.5b03730

Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling. / Park, Mi Ri; Oh, Sangnam; Son, Seok Jun; Park, Dong June; Oh, Sejong; Kim, Sae Hun; Jeong, Do Youn; Oh, Nam Su; Lee, Youngbok; Song, Minho; Kim, Younghoon.

In: Journal of agricultural and food chemistry, Vol. 63, No. 47, 06.11.2015, p. 10227-10233.

Research output: Contribution to journal › Article › peer-review

Park, MR, Oh, S, Son, SJ, Park, DJ, Oh, S, Kim, SH, Jeong, DY, Oh, NS, Lee, Y, Song, M & Kim, Y 2015, 'Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling', Journal of agricultural and food chemistry, vol. 63, no. 47, pp. 10227-10233. https://doi.org/10.1021/acs.jafc.5b03730

Park, Mi Ri ; Oh, Sangnam ; Son, Seok Jun ; Park, Dong June ; Oh, Sejong ; Kim, Sae Hun ; Jeong, Do Youn ; Oh, Nam Su ; Lee, Youngbok ; Song, Minho ; Kim, Younghoon. / Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling. In: Journal of agricultural and food chemistry. 2015 ; Vol. 63, No. 47. pp. 10227-10233.

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abstract = "In this study, we investigated potentially probiotic Bacillus licheniformis strains isolated from traditional Korean food sources for ability to enhance longevity using the nematode Caenorhabditis elegans as a simple in vivo animal model. We first investigated whether B. licheniformis strains were capable of modulating the lifespan of C. elegans. Among the tested strains, preconditioning with four B. licheniformis strains significantly enhanced the longevity of C. elegans. Unexpectedly, plate counting and transmission electron microscopy (TEM) results indicated that B. licheniformis strains were not more highly attached to the C. elegans intestine compared with Escherichia coli OP50 or Lactobacillus rhamnosus GG controls. In addition, qRT-PCR and an aging assay with mutant worms showed that the conditioning of B. licheniformis strain 141 directly influenced genes associated with serotonin signaling in nematodes, including tph-1 (tryptophan hydroxylase), bas-1 (serotonin- and dopamine-synthetic aromatic amino acid decarboxylase), mod-1 (serotonin-gated chloride channel), ser-1, and ser-7 (serotonin receptors) during C. elegans aging. Our findings suggest that B. licheniformis strain 141, which is isolated from traditional Korean foods, is a probiotic generally recognized as safe (GRAS) strain that enhances the lifespan of C. elegans via host serotonin signaling.",

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AU - Kim, Sae Hun

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AU - Song, Minho

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Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling

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