Barley intake induces bile acid excretion by reduced expression of intestinal ASBT and NPC1L1 in C57BL/6J mice

Minh Hien Hoang, Soung Jin Houng, Hee Jin Jun, Ji Hae Lee, Jin Woong Choi, So Hee Kim, Yong Ro Kim, Sung Joon Lee

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

To investigate the hypocholesterolemic mechanism of barley in vivo, six-week-old C57BL/6J mice were fed a high-fat diet (HFD) or high-fat diet containing barley (HFD-B) for seven weeks. Total and LDL cholesterol concentrations were significantly reduced in the HFD-B group while fecal cholesterol and bile acid was increased. Real-time PCR and immunoblot analysis revealed the induction of FXR expression, which in turn suppressed the expression of ASBT and NPC1L1 in the HFD-B group compared with the controls. In the liver, the expression of HMG-CoA reductase was significantly reduced while LDL receptor expression was unaltered in the HFD-B group compared with the controls. Our data suggest that the hypocholesterolemic effects of barley are primarily the result of reduced dietary cholesterol uptake and bile acid resorption. Reduced expression of intestinal ASBT and NPC1L1 may play a key role in the regulation of dietary cholesterol and bile acid metabolism in mice consuming a diet containing barley.

Original languageEnglish
Pages (from-to)6798-6805
Number of pages8
JournalJournal of agricultural and food chemistry
Volume59
Issue number12
DOIs
Publication statusPublished - 2011 Jun 22

Keywords

  • ASBT
  • NPC1L1
  • barley
  • bile acid
  • cholesterol

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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