Barley sprout extract containing policosanols and polyphenols regulate AMPK, SREBP2 and ACAT2 activity and cholesterol and glucose metabolism in vitro and in vivo

Ji Hae Lee, So Young Lee, Bobae Kim, Woo Duck Seo, Yaoyao Jia, Chunyan Wu, Hee jin Jun, Sung-Joon Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Mechanism of barley sprout extract (BS), which contains policosanols and polyphenols, on cholesterol and glucose metabolism was investigated. BS reduced the intracellular cholesterol concentrations in the HepG2 cells and the plasma cholesterol concentrations in the mice by the activation of AMPK and the subsequent phosphorylation inhibition of HMGCR. BS suppressed the nuclear translocation of SREBP2, reducing the transcription of HMGCR. AMPK activation with BS reduced the fasting glucose and hepatic triglyceride concentrations in mice by repressing the hepatic gluconeogenic genes, including fructose-1, 6-bisphosphatase and pyruvate carboxylase and the plasma levels of the proinflammatory cytokines tumor necrosis factor-α and interleukin-6. The activation of hepatic autophagy by BS was confirmed by induced protein expressions of LC3-II and LAMP. In conclusion, BS activates AMPK and hepatic autophagy and inhibits SREBP2, resulting in hypocholesterolemic and hypoglycemic activities and improvements in the symptoms of hepatic steatosis.

Original languageEnglish
Pages (from-to)174-183
Number of pages10
JournalFood Research International
Volume72
DOIs
Publication statusPublished - 2015 Jun 1

Keywords

  • 3-Hydroxy-3-methyl-glutaryl-CoA reductase
  • 5'-Adenosine monophosphate activated protein kinase
  • Autophagy
  • Barley sprout extract
  • Cholesterol metabolism

ASJC Scopus subject areas

  • Food Science

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