Abstract
Mechanism of barley sprout extract (BS), which contains policosanols and polyphenols, on cholesterol and glucose metabolism was investigated. BS reduced the intracellular cholesterol concentrations in the HepG2 cells and the plasma cholesterol concentrations in the mice by the activation of AMPK and the subsequent phosphorylation inhibition of HMGCR. BS suppressed the nuclear translocation of SREBP2, reducing the transcription of HMGCR. AMPK activation with BS reduced the fasting glucose and hepatic triglyceride concentrations in mice by repressing the hepatic gluconeogenic genes, including fructose-1, 6-bisphosphatase and pyruvate carboxylase and the plasma levels of the proinflammatory cytokines tumor necrosis factor-α and interleukin-6. The activation of hepatic autophagy by BS was confirmed by induced protein expressions of LC3-II and LAMP. In conclusion, BS activates AMPK and hepatic autophagy and inhibits SREBP2, resulting in hypocholesterolemic and hypoglycemic activities and improvements in the symptoms of hepatic steatosis.
| Original language | English |
|---|---|
| Pages (from-to) | 174-183 |
| Number of pages | 10 |
| Journal | Food Research International |
| Volume | 72 |
| DOIs | |
| Publication status | Published - 2015 Jun 1 |
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Keywords
- 3-Hydroxy-3-methyl-glutaryl-CoA reductase
- 5'-Adenosine monophosphate activated protein kinase
- Autophagy
- Barley sprout extract
- Cholesterol metabolism
ASJC Scopus subject areas
- Food Science
Cite this
Barley sprout extract containing policosanols and polyphenols regulate AMPK, SREBP2 and ACAT2 activity and cholesterol and glucose metabolism in vitro and in vivo. / Lee, Ji Hae; Lee, So Young; Kim, Bobae; Seo, Woo Duck; Jia, Yaoyao; Wu, Chunyan; Jun, Hee jin; Lee, Sung-Joon.
In: Food Research International, Vol. 72, 01.06.2015, p. 174-183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Barley sprout extract containing policosanols and polyphenols regulate AMPK, SREBP2 and ACAT2 activity and cholesterol and glucose metabolism in vitro and in vivo
AU - Lee, Ji Hae
AU - Lee, So Young
AU - Kim, Bobae
AU - Seo, Woo Duck
AU - Jia, Yaoyao
AU - Wu, Chunyan
AU - Jun, Hee jin
AU - Lee, Sung-Joon
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Mechanism of barley sprout extract (BS), which contains policosanols and polyphenols, on cholesterol and glucose metabolism was investigated. BS reduced the intracellular cholesterol concentrations in the HepG2 cells and the plasma cholesterol concentrations in the mice by the activation of AMPK and the subsequent phosphorylation inhibition of HMGCR. BS suppressed the nuclear translocation of SREBP2, reducing the transcription of HMGCR. AMPK activation with BS reduced the fasting glucose and hepatic triglyceride concentrations in mice by repressing the hepatic gluconeogenic genes, including fructose-1, 6-bisphosphatase and pyruvate carboxylase and the plasma levels of the proinflammatory cytokines tumor necrosis factor-α and interleukin-6. The activation of hepatic autophagy by BS was confirmed by induced protein expressions of LC3-II and LAMP. In conclusion, BS activates AMPK and hepatic autophagy and inhibits SREBP2, resulting in hypocholesterolemic and hypoglycemic activities and improvements in the symptoms of hepatic steatosis.
AB - Mechanism of barley sprout extract (BS), which contains policosanols and polyphenols, on cholesterol and glucose metabolism was investigated. BS reduced the intracellular cholesterol concentrations in the HepG2 cells and the plasma cholesterol concentrations in the mice by the activation of AMPK and the subsequent phosphorylation inhibition of HMGCR. BS suppressed the nuclear translocation of SREBP2, reducing the transcription of HMGCR. AMPK activation with BS reduced the fasting glucose and hepatic triglyceride concentrations in mice by repressing the hepatic gluconeogenic genes, including fructose-1, 6-bisphosphatase and pyruvate carboxylase and the plasma levels of the proinflammatory cytokines tumor necrosis factor-α and interleukin-6. The activation of hepatic autophagy by BS was confirmed by induced protein expressions of LC3-II and LAMP. In conclusion, BS activates AMPK and hepatic autophagy and inhibits SREBP2, resulting in hypocholesterolemic and hypoglycemic activities and improvements in the symptoms of hepatic steatosis.
KW - 3-Hydroxy-3-methyl-glutaryl-CoA reductase
KW - 5'-Adenosine monophosphate activated protein kinase
KW - Autophagy
KW - Barley sprout extract
KW - Cholesterol metabolism
UR - http://www.scopus.com/inward/record.url?scp=84927641018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84927641018&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2015.03.041
DO - 10.1016/j.foodres.2015.03.041
M3 - Article
AN - SCOPUS:84927641018
VL - 72
SP - 174
EP - 183
JO - Food Research International
JF - Food Research International
SN - 0963-9969
ER -