Base-pair opening dynamics of the microRNA precursor pri-miR156a affect temperature-responsive flowering in Arabidopsis

Hee Eun Kim, Wanhui Kim, Ae Ree Lee, Suhyun Jin, A. Rim Jun, Nak Kyoon Kim, Joon Hwa Lee, Ji Hoon Ahn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Internal and environmental cues, including ambient temperature changes, regulate the timing of flowering in plants. Arabidopsis miR156 represses flowering and plays an important role in the regulation of temperature-responsive flowering. However, the molecular basis of miR156 processing at lower temperatures remains largely unknown. Here, we performed nuclear magnetic resonance studies to investigate the base-pair opening dynamics of model RNAs at 16 °C and investigated the in vivo effects of the mutant RNAs on temperature-responsive flowering. The A9C and A10CG mutations in the B5 bulge of the lower stem of pri-miR156a stabilized the C15∙G98 and U16∙A97 base-pairs at the cleavage site of pri-miR156a at 16 °C. Consistent with this, production of mature miR156 was severely affected in plants overexpressing the A9C and A10CG constructs and these plants exhibited almost no delay in flowering at 16 °C. The A10G and A9AC mutations did not strongly affect C15∙G98 and U16∙A97 base-pairs at 16 °C, and plants overexpressing A10G and A9AC mutants of miR156 produced more mature miR156 than plants overexpressing the A9C and A10CG mutants and showed a strong delay in flowering at 16 °C. Interestingly, the A9AC mutation had distinct effects on the opening dynamics of the C15∙G98 and U16∙A97 base-pairs between 16 °C and 23 °C, and plants expressing the A9AC mutant miR156 showed only a moderate delay in flowering at 16 °C. Based on these results, we propose that fine-tuning of the base-pair stability at the cleavage site is essential for efficient processing of pri-miR156a at a low temperature and for reduced flowering sensitivity to ambient temperature changes.

Original languageEnglish
Pages (from-to)839-844
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume484
Issue number4
DOIs
Publication statusPublished - 2017 Mar 18

Fingerprint

MicroRNAs
Arabidopsis
Base Pairing
Temperature
Mutation
RNA
Processing
Cues
Magnetic Resonance Spectroscopy
Tuning
Nuclear magnetic resonance

Keywords

  • Base-pair opening dynamics
  • Flowering
  • Microrna biogenesis
  • NMR
  • RNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Base-pair opening dynamics of the microRNA precursor pri-miR156a affect temperature-responsive flowering in Arabidopsis. / Kim, Hee Eun; Kim, Wanhui; Lee, Ae Ree; Jin, Suhyun; Jun, A. Rim; Kim, Nak Kyoon; Lee, Joon Hwa; Ahn, Ji Hoon.

In: Biochemical and Biophysical Research Communications, Vol. 484, No. 4, 18.03.2017, p. 839-844.

Research output: Contribution to journalArticle

Kim, Hee Eun ; Kim, Wanhui ; Lee, Ae Ree ; Jin, Suhyun ; Jun, A. Rim ; Kim, Nak Kyoon ; Lee, Joon Hwa ; Ahn, Ji Hoon. / Base-pair opening dynamics of the microRNA precursor pri-miR156a affect temperature-responsive flowering in Arabidopsis. In: Biochemical and Biophysical Research Communications. 2017 ; Vol. 484, No. 4. pp. 839-844.
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AU - Jin, Suhyun

AU - Jun, A. Rim

AU - Kim, Nak Kyoon

AU - Lee, Joon Hwa

AU - Ahn, Ji Hoon

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