Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells

Jin Won Choi, Sujung Kim, Tae Min Kim, Young Min Kim, Hee Won Seo, Tae Sub Park, Jae Wook Jeong, Gwonhwa Song, Jae Yong Han

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Background: Long-term maintenance of avian primordial germ cells (PGCs) in vitro has tremendous potential because it can be used to deepen our understanding of the biology of PGCs. A transgenic bioreactor based on the unique migration of PGCs toward the recipients' sex cord via the bloodstream and thereby creating a germline chimeric bird has many potential applications. However, the growth factors and the signaling pathway essential for inducing proliferation of chicken PGCs are unknown. Methodology/Principal Findings: Therefore, we conducted this study to investigate the effects of various combinations of growth factors on the survival and proliferation of PGCs under feeder-free conditions. We observed proliferation of PGCs in media containing bFGF. Subsequent characterization confirmed that the cultured PGCs maintained expression of PGC-specific markers, telomerase activity, normal migrational activity, and germline transmission. We also found that bFGF activates the mitogen-activated protein kinase kinase/extracellular-signal regulated kinase (MEK/ERK) signaling. Also, the expression of 133 transcripts was reversibly altered by bFGF withdrawal. Conclusions/Significance: Our results demonstrate that chicken PGCs can be maintained in vitro without any differentiation or dedifferentiation in feeder free culture conditions, and subsequent analysis revealed that bFGF is one of the key factors that enable proliferation of chicken PGCs via MEK/ERK signaling regulating downstream genes that may be important for PGC proliferation and survival.

Original languageEnglish
Article numbere12968
JournalPLoS One
Volume5
Issue number9
DOIs
Publication statusPublished - 2010 Nov 1
Externally publishedYes

Fingerprint

Cell signaling
mitogen-activated protein kinase kinase
fibroblast growth factor 2
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Fibroblast Growth Factor 2
mitogen-activated protein kinase
Germ Cells
Chickens
germ cells
Cells
chickens
Intercellular Signaling Peptides and Proteins
cells
Telomerase
Cell proliferation
Birds
Bioreactors
Genes
growth factors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells. / Choi, Jin Won; Kim, Sujung; Kim, Tae Min; Kim, Young Min; Seo, Hee Won; Park, Tae Sub; Jeong, Jae Wook; Song, Gwonhwa; Han, Jae Yong.

In: PLoS One, Vol. 5, No. 9, e12968, 01.11.2010.

Research output: Contribution to journalArticle

Choi, Jin Won ; Kim, Sujung ; Kim, Tae Min ; Kim, Young Min ; Seo, Hee Won ; Park, Tae Sub ; Jeong, Jae Wook ; Song, Gwonhwa ; Han, Jae Yong. / Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells. In: PLoS One. 2010 ; Vol. 5, No. 9.
@article{e676c85c34be4972b204e89797b9f8f3,
title = "Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells",
abstract = "Background: Long-term maintenance of avian primordial germ cells (PGCs) in vitro has tremendous potential because it can be used to deepen our understanding of the biology of PGCs. A transgenic bioreactor based on the unique migration of PGCs toward the recipients' sex cord via the bloodstream and thereby creating a germline chimeric bird has many potential applications. However, the growth factors and the signaling pathway essential for inducing proliferation of chicken PGCs are unknown. Methodology/Principal Findings: Therefore, we conducted this study to investigate the effects of various combinations of growth factors on the survival and proliferation of PGCs under feeder-free conditions. We observed proliferation of PGCs in media containing bFGF. Subsequent characterization confirmed that the cultured PGCs maintained expression of PGC-specific markers, telomerase activity, normal migrational activity, and germline transmission. We also found that bFGF activates the mitogen-activated protein kinase kinase/extracellular-signal regulated kinase (MEK/ERK) signaling. Also, the expression of 133 transcripts was reversibly altered by bFGF withdrawal. Conclusions/Significance: Our results demonstrate that chicken PGCs can be maintained in vitro without any differentiation or dedifferentiation in feeder free culture conditions, and subsequent analysis revealed that bFGF is one of the key factors that enable proliferation of chicken PGCs via MEK/ERK signaling regulating downstream genes that may be important for PGC proliferation and survival.",
author = "Choi, {Jin Won} and Sujung Kim and Kim, {Tae Min} and Kim, {Young Min} and Seo, {Hee Won} and Park, {Tae Sub} and Jeong, {Jae Wook} and Gwonhwa Song and Han, {Jae Yong}",
year = "2010",
month = "11",
day = "1",
doi = "10.1371/journal.pone.0012968",
language = "English",
volume = "5",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Basic fibroblast growth factor activates MEK/ERK cell signaling pathway and stimulates the proliferation of chicken primordial germ cells

AU - Choi, Jin Won

AU - Kim, Sujung

AU - Kim, Tae Min

AU - Kim, Young Min

AU - Seo, Hee Won

AU - Park, Tae Sub

AU - Jeong, Jae Wook

AU - Song, Gwonhwa

AU - Han, Jae Yong

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Background: Long-term maintenance of avian primordial germ cells (PGCs) in vitro has tremendous potential because it can be used to deepen our understanding of the biology of PGCs. A transgenic bioreactor based on the unique migration of PGCs toward the recipients' sex cord via the bloodstream and thereby creating a germline chimeric bird has many potential applications. However, the growth factors and the signaling pathway essential for inducing proliferation of chicken PGCs are unknown. Methodology/Principal Findings: Therefore, we conducted this study to investigate the effects of various combinations of growth factors on the survival and proliferation of PGCs under feeder-free conditions. We observed proliferation of PGCs in media containing bFGF. Subsequent characterization confirmed that the cultured PGCs maintained expression of PGC-specific markers, telomerase activity, normal migrational activity, and germline transmission. We also found that bFGF activates the mitogen-activated protein kinase kinase/extracellular-signal regulated kinase (MEK/ERK) signaling. Also, the expression of 133 transcripts was reversibly altered by bFGF withdrawal. Conclusions/Significance: Our results demonstrate that chicken PGCs can be maintained in vitro without any differentiation or dedifferentiation in feeder free culture conditions, and subsequent analysis revealed that bFGF is one of the key factors that enable proliferation of chicken PGCs via MEK/ERK signaling regulating downstream genes that may be important for PGC proliferation and survival.

AB - Background: Long-term maintenance of avian primordial germ cells (PGCs) in vitro has tremendous potential because it can be used to deepen our understanding of the biology of PGCs. A transgenic bioreactor based on the unique migration of PGCs toward the recipients' sex cord via the bloodstream and thereby creating a germline chimeric bird has many potential applications. However, the growth factors and the signaling pathway essential for inducing proliferation of chicken PGCs are unknown. Methodology/Principal Findings: Therefore, we conducted this study to investigate the effects of various combinations of growth factors on the survival and proliferation of PGCs under feeder-free conditions. We observed proliferation of PGCs in media containing bFGF. Subsequent characterization confirmed that the cultured PGCs maintained expression of PGC-specific markers, telomerase activity, normal migrational activity, and germline transmission. We also found that bFGF activates the mitogen-activated protein kinase kinase/extracellular-signal regulated kinase (MEK/ERK) signaling. Also, the expression of 133 transcripts was reversibly altered by bFGF withdrawal. Conclusions/Significance: Our results demonstrate that chicken PGCs can be maintained in vitro without any differentiation or dedifferentiation in feeder free culture conditions, and subsequent analysis revealed that bFGF is one of the key factors that enable proliferation of chicken PGCs via MEK/ERK signaling regulating downstream genes that may be important for PGC proliferation and survival.

UR - http://www.scopus.com/inward/record.url?scp=77958605259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958605259&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0012968

DO - 10.1371/journal.pone.0012968

M3 - Article

C2 - 20886037

AN - SCOPUS:77958605259

VL - 5

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e12968

ER -