Battery operated preconcentration-assisted lateral flow assay

Cheonjung Kim, Yong Kyoung Yoo, Sung Il Han, Junwoo Lee, Dohwan Lee, Kyungjae Lee, Kyo Seon Hwang, Kyu Hyoung Lee, Seok Chung, Jeong Hoon Lee

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Paper-based analytical devices (e.g. lateral flow assays) are highly advantageous as portable diagnostic systems owing to their low costs and ease of use. Because of their low sensitivity and detection limits for biomolecules, these devices have several limitations in applications for real-field diagnosis. Here, we demonstrate a paper-based preconcentration enhanced lateral flow assay using a commercial β-hCG-based test. Utilizing a simple 9 V battery operation with a low power consumption of approximately 81 μW, we acquire a 25-fold preconcentration factor, demonstrating a clear sensitivity enhancement in the colorimetric lateral flow assay; consequently, clear colors are observed in a rapid kit test line, which cannot be monitored without preconcentration. This device can also facilitate a semi-quantitative platform using the saturation value and/or color intensity in both paper-based colorimetric assays and smartphone-based diagnostics.

Original languageEnglish
Pages (from-to)2451-2458
Number of pages8
JournalLab on a chip
Volume17
Issue number14
DOIs
Publication statusPublished - 2017

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biochemistry
  • Biomedical Engineering

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