Bax-dependent and -independent death of motoneurons after facial nerve injury in adult mice

Ok Hee Park, Kea Joo Lee, Im Joo Rhyu, Dongho Geum, Hyun Kim, Robert Buss, Ronald W. Oppenheim, Woong Sun

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Nerve injury-induced neuronal death may occur after accidental trauma or nerve inflammation. Although the response to facial root avulsion has been examined in rodent models, there are conflicting results as to whether motoneuron (MN) death is mediated by apoptosis or necrosis. We examined the response of MNs and proximal nerves after facial nerve avulsion in adult mice. Following facial nerve avulsion in 4-5-week-old mice, we observed a progressive reduction of MNs such that by 4 weeks less than 10% of avulsed MNs remained compared with the control side. The profile of MN degeneration was distinct from axotomy-induced responses. For example, the onset of MN death was more rapid, and the extent of MN loss was greater compared with axotomy. Furthermore, the degeneration of oligodendrocytes and the activation of microglia were increased in the proximal nerve after avulsion. Ultrastructural observations suggested that root avulsion mainly induces non-apoptotic neuronal death, although a small subset of neurons appeared to die via apoptosis. To evaluate the contribution of apoptotic death, we evaluated MN responses in Bax-knockout (KO) mice in which neurons are rescued from apoptotic death. Surprisingly, although the majority of Bax-KO mice exhibited only a moderate MN loss after avulsion, a subset of Bax-KO mice (25%) exhibited extensive MN death and injury-induced changes in the nerve that were indistinguishable from events in wild-type littermates. These results suggest that both Bax-dependent and -independent forms of cell death are evoked by root avulsion, and that programmed cell death may be involved in triggering a robust necrotic response.

Original languageEnglish
Pages (from-to)1421-1432
Number of pages12
JournalEuropean Journal of Neuroscience
Volume26
Issue number6
DOIs
Publication statusPublished - 2007 Sep

Keywords

  • Cell death
  • Microglia
  • Oligodendrocyte
  • Root avulsion

ASJC Scopus subject areas

  • Neuroscience(all)

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