Abstract
Autoimmune type 1 diabetes mellitus is caused by the immunologic destruction of pancreatic β-cells; therefore, there have been many attempts at immunologic modulation as a block or prevention of the underlying process. The aim of this study is to investigate the effect of BCG vaccination on low dose streptozotocin-induced diabetic (LDSD) mice. The mice were pretreated with BCG 7 days before starting low dose streptozotocin (STZ), observed body weight and blood glucose for 2 months, then analyzed the severity of the STZ- induced insulitis after the animals were sacrificed. In this experiment, the mean body weights in the BCG-STZ group on days 1, 19, 33, 47, and 61 of the experiment were 37.5 ± 3.6, 37.3 ± 3.6, 37.5 ± 3.5, 39.4 ± 3.9, and 39.3 ± 4.5 (g), respectively. Those in the STZ group were 37.7 ± 3.5, 38.3 ± 4.5, 38.4 ± 3.9, 36.2 ± 4.5, and 36.3 ± 4.0 (g), respectively (P < 0.05). The mean blood glucose levels in the BCG-STZ group on days 1, 19, 33, 47, and 61 were 106.5 ± 8, 150 ± 37, 147 ± 54, 143 ± 60, and 142 ± 66 (mg/dl), respectively. Those in the STZ group were 103 ± 12, 196 ± 90, 261 ± 236, 236 ± 91, and 224 ± 89 (mg/dl), respectively (P < 0.05). The numbers developing grade 0, I, II, III, and IV insulitis in the BCG-treated group were 63, 48, 33, 4, and 2, respectively, and in the control group were 16, 23, 31, 45, and 35, respectively. This study indicates that BCG vaccination reduces the development of insulitis and overt diabetes in LDSD mice.
| Original language | English |
|---|---|
| Pages (from-to) | 91-97 |
| Number of pages | 7 |
| Journal | Diabetes Research and Clinical Practice |
| Volume | 46 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 Nov 1 |
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Keywords
- Autoimmune type 1 diabetes mellitus
- BCG
- Insulitis
- LDSD
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
- Endocrinology
Cite this
BCG vaccine prevents insulitis in low dose streptozotocin-induced diabetic mice. / Baik, Sei-Hyun; Park, Ie Byung; Choi, Kyung Mook; Kim, Yong Hyun; Kim, Nan Hee; Kim, Sang Jin; Song, Gwan Gyu; Choi, Dong Seop.
In: Diabetes Research and Clinical Practice, Vol. 46, No. 2, 01.11.1999, p. 91-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BCG vaccine prevents insulitis in low dose streptozotocin-induced diabetic mice
AU - Baik, Sei-Hyun
AU - Park, Ie Byung
AU - Choi, Kyung Mook
AU - Kim, Yong Hyun
AU - Kim, Nan Hee
AU - Kim, Sang Jin
AU - Song, Gwan Gyu
AU - Choi, Dong Seop
PY - 1999/11/1
Y1 - 1999/11/1
N2 - Autoimmune type 1 diabetes mellitus is caused by the immunologic destruction of pancreatic β-cells; therefore, there have been many attempts at immunologic modulation as a block or prevention of the underlying process. The aim of this study is to investigate the effect of BCG vaccination on low dose streptozotocin-induced diabetic (LDSD) mice. The mice were pretreated with BCG 7 days before starting low dose streptozotocin (STZ), observed body weight and blood glucose for 2 months, then analyzed the severity of the STZ- induced insulitis after the animals were sacrificed. In this experiment, the mean body weights in the BCG-STZ group on days 1, 19, 33, 47, and 61 of the experiment were 37.5 ± 3.6, 37.3 ± 3.6, 37.5 ± 3.5, 39.4 ± 3.9, and 39.3 ± 4.5 (g), respectively. Those in the STZ group were 37.7 ± 3.5, 38.3 ± 4.5, 38.4 ± 3.9, 36.2 ± 4.5, and 36.3 ± 4.0 (g), respectively (P < 0.05). The mean blood glucose levels in the BCG-STZ group on days 1, 19, 33, 47, and 61 were 106.5 ± 8, 150 ± 37, 147 ± 54, 143 ± 60, and 142 ± 66 (mg/dl), respectively. Those in the STZ group were 103 ± 12, 196 ± 90, 261 ± 236, 236 ± 91, and 224 ± 89 (mg/dl), respectively (P < 0.05). The numbers developing grade 0, I, II, III, and IV insulitis in the BCG-treated group were 63, 48, 33, 4, and 2, respectively, and in the control group were 16, 23, 31, 45, and 35, respectively. This study indicates that BCG vaccination reduces the development of insulitis and overt diabetes in LDSD mice.
AB - Autoimmune type 1 diabetes mellitus is caused by the immunologic destruction of pancreatic β-cells; therefore, there have been many attempts at immunologic modulation as a block or prevention of the underlying process. The aim of this study is to investigate the effect of BCG vaccination on low dose streptozotocin-induced diabetic (LDSD) mice. The mice were pretreated with BCG 7 days before starting low dose streptozotocin (STZ), observed body weight and blood glucose for 2 months, then analyzed the severity of the STZ- induced insulitis after the animals were sacrificed. In this experiment, the mean body weights in the BCG-STZ group on days 1, 19, 33, 47, and 61 of the experiment were 37.5 ± 3.6, 37.3 ± 3.6, 37.5 ± 3.5, 39.4 ± 3.9, and 39.3 ± 4.5 (g), respectively. Those in the STZ group were 37.7 ± 3.5, 38.3 ± 4.5, 38.4 ± 3.9, 36.2 ± 4.5, and 36.3 ± 4.0 (g), respectively (P < 0.05). The mean blood glucose levels in the BCG-STZ group on days 1, 19, 33, 47, and 61 were 106.5 ± 8, 150 ± 37, 147 ± 54, 143 ± 60, and 142 ± 66 (mg/dl), respectively. Those in the STZ group were 103 ± 12, 196 ± 90, 261 ± 236, 236 ± 91, and 224 ± 89 (mg/dl), respectively (P < 0.05). The numbers developing grade 0, I, II, III, and IV insulitis in the BCG-treated group were 63, 48, 33, 4, and 2, respectively, and in the control group were 16, 23, 31, 45, and 35, respectively. This study indicates that BCG vaccination reduces the development of insulitis and overt diabetes in LDSD mice.
KW - Autoimmune type 1 diabetes mellitus
KW - BCG
KW - Insulitis
KW - LDSD
UR - http://www.scopus.com/inward/record.url?scp=0344069775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344069775&partnerID=8YFLogxK
U2 - 10.1016/S0168-8227(99)00079-0
DO - 10.1016/S0168-8227(99)00079-0
M3 - Article
VL - 46
SP - 91
EP - 97
JO - Diabetes Research and Clinical Practice
JF - Diabetes Research and Clinical Practice
SN - 0168-8227
IS - 2
ER -