Bead based facile assay for sensitive quantification of native state green fluorescent protein

Jung Min Kim; Baik Lin Seong; Dong Kwon Lim

doi:10.1039/c9ra09599c

Bead based facile assay for sensitive quantification of native state green fluorescent protein

Jung Min Kim, Baik Lin Seong, Dong Kwon Lim

Research output: Contribution to journal › Article › peer-review

Abstract

A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

Original language	English
Pages (from-to)	13095-13099
Number of pages	5
Journal	RSC Advances
Volume	10
Issue number	22
DOIs	https://doi.org/10.1039/c9ra09599c
Publication status	Published - 2020 Mar 31

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.1039/c9ra09599c

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title = "Bead based facile assay for sensitive quantification of native state green fluorescent protein",

abstract = "A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.",

author = "Kim, {Jung Min} and Seong, {Baik Lin} and Lim, {Dong Kwon}",

note = "Funding Information: This research was supported by Basic Science Research Program and through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018M3A9H4079358) and (NRF-2019R1I1A1A01043047). This work was also supported by a Korea University Grant, NRF-2017M3D1A1039421, and KU-KIST school research fund. Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

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N2 - A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

AB - A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

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Bead based facile assay for sensitive quantification of native state green fluorescent protein

Abstract

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