Bead based facile assay for sensitive quantification of native state green fluorescent protein

Jung Min Kim, Baik Lin Seong, Dong Kwon Lim

Research output: Contribution to journalArticle

Abstract

A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

Original languageEnglish
Pages (from-to)13095-13099
Number of pages5
JournalRSC Advances
Volume10
Issue number22
DOIs
Publication statusPublished - 2020 Mar 31

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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