Abstract
A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.
| Original language | English |
|---|---|
| Pages (from-to) | 13095-13099 |
| Number of pages | 5 |
| Journal | RSC Advances |
| Volume | 10 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 2020 Mar 31 |
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
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Cite this
Kim, J. M., Seong, B. L., & Lim, D. K. (2020). Bead based facile assay for sensitive quantification of native state green fluorescent protein. RSC Advances, 10(22), 13095-13099. https://doi.org/10.1039/c9ra09599c