Abstract
A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.
Original language | English |
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Pages (from-to) | 13095-13099 |
Number of pages | 5 |
Journal | RSC Advances |
Volume | 10 |
Issue number | 22 |
DOIs | |
Publication status | Published - 2020 Mar 31 |
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)