Behavior and differentiation studies of hASCs and rBMSCs by the γ-ray irradiation

Ji Eun Kim, Soo Hyun Kim, Jae Hoon Jeong, Young Hoon Ji, Youngmee Jung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recently, in tissue engineering, ionizing radiation has been used as stimulation. Especially, several studies showed that low-dose ionizing radiation induced the stimulation of cell proliferation. Also, it is reported that single low dose γ-irradiation for whole-body or lymphoid-organ significantly improved the spontaneous recovery. The purpose of this study is to investigate behavior and differentiation of human adipose tissue-derived stem cells (hASCs) and rabbit bone marrow stromal cells (rBMSCs) after low dose and high dose of γ-irradiation. Cells were irradiated with γ-rays of 1, 10, 100 mGy and 1 Gy. In addition, we investigated the effects on the osteogenic differentiation of γ-irradiation. PLLA scaffolds with 90% porosity and 300-500 μm pore size were fabricated and cells were seeded onto the scaffolds. After that, cell-scaffold construct were irradiated with 1 mGy and implanted subcutaneously in nude mice for up to 10 weeks. Specimens were harvested after 10 weeks and analyzed. In the results, p-H2AX/DAPI ratio increased with time while the p-H2X expression was not found all time point in control (no irradiation) group. But, in the group radiated with low γ-ray (1 mGy), the p-H2X expression almost did not appear similar with non-radiated group. However, in the osteogenic differentiation tests, the control group and the irradiated group revealed no significant difference. In conclusion, low dose γ-irradiation could be used as stimulation for tissue engineering using specific stem cells and consequently, this system will be useful for combination technology of radiation and cell therapy. However, further studies are needed for examining the general mechanism of the effects on the low dose irradiation for stem cells.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalTissue Engineering and Regenerative Medicine
Volume11
Issue numberS1
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Dosimetry
Adipose Tissue
Bone
Stem Cells
Irradiation
Tissue Engineering
Tissue
Rabbits
Ionizing Radiation
Scaffolds
Whole-Body Irradiation
Porosity
Ionizing radiation
Cell- and Tissue-Based Therapy
Tissue engineering
Nude Mice
Radiotherapy
Cell Proliferation

Keywords

  • adipose tissue-derived stem cells
  • bone marrow derived stem cells
  • osteogenic differentiation
  • stimulation
  • γ-irradiation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Behavior and differentiation studies of hASCs and rBMSCs by the γ-ray irradiation. / Kim, Ji Eun; Kim, Soo Hyun; Jeong, Jae Hoon; Ji, Young Hoon; Jung, Youngmee.

In: Tissue Engineering and Regenerative Medicine, Vol. 11, No. S1, 01.01.2014, p. 24-31.

Research output: Contribution to journalArticle

Kim, Ji Eun ; Kim, Soo Hyun ; Jeong, Jae Hoon ; Ji, Young Hoon ; Jung, Youngmee. / Behavior and differentiation studies of hASCs and rBMSCs by the γ-ray irradiation. In: Tissue Engineering and Regenerative Medicine. 2014 ; Vol. 11, No. S1. pp. 24-31.
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