Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats

Hoon Chul Kang, Dae-Sung Kim, Ji Young Kim, Han Soo Kim, Bo Young Lim, Heung Dong Kim, Jin Sung Lee, Baik-Lin Eun, Dong Wook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: We intended to determine whether transplanted neural precursors, derived from mouse embryonic stem (ES) cells, can migrate and differentiate into mature neurons and glial cells in damaged brains and improve functional deficits caused by global cerebral ischemic injury in adolescent rats. Methods: Global ischemia was induced using the four-vessel occlusion method. ES cells that display enhanced expression of yellow fluorescent protein were co-cultured in N2 supplemented media with PA6 cells that had stromal derived inducing activity. Neural precursor cells were directly transplanted bilaterally into hippocampal C3 areas 2. weeks after induction of global ischemia. Assessments of the Morris water-maze test at eight weeks and, the Open field activity levels at two, four, six and eight weeks after transplantation were carried out according to standard methods. Results: From neural precursors, we were able to generate neural lineages, including neurons and glial cells in vitro. Eight weeks following transplantation, cellular migration as well as generation of neural cells including neurons, astrocytes, and oligodendrocytes developed from the grafted ES cell-derived neural precursors were observed. Cell-transplanted animals exhibited enhanced functional recovery on sensorimotor and behavioral tests, compared to vehicle-treated control animals. Conclusion: Therefore, transplantation of mouse ES cell-derived neural precursor cells shows promise for improving recovery after global ischemia in adolescent rats.

Original languageEnglish
Pages (from-to)658-668
Number of pages11
JournalBrain and Development
Volume32
Issue number8
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Embryonic Stem Cells
Transplantation
Ischemia
Brain
Neurons
Neuroglia
Oligodendroglia
Stromal Cells
Astrocytes
Water
Wounds and Injuries
Proteins
Mouse Embryonic Stem Cells

Keywords

  • Cell transplantation
  • Embryonic stem cells
  • Globally ischemic brain
  • Neural precursors

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Developmental Neuroscience
  • Clinical Neurology

Cite this

Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats. / Kang, Hoon Chul; Kim, Dae-Sung; Kim, Ji Young; Kim, Han Soo; Lim, Bo Young; Kim, Heung Dong; Lee, Jin Sung; Eun, Baik-Lin; Kim, Dong Wook.

In: Brain and Development, Vol. 32, No. 8, 01.09.2010, p. 658-668.

Research output: Contribution to journalArticle

Kang, Hoon Chul ; Kim, Dae-Sung ; Kim, Ji Young ; Kim, Han Soo ; Lim, Bo Young ; Kim, Heung Dong ; Lee, Jin Sung ; Eun, Baik-Lin ; Kim, Dong Wook. / Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats. In: Brain and Development. 2010 ; Vol. 32, No. 8. pp. 658-668.
@article{5bce467c8ade4e299413ccc92aee93c0,
title = "Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats",
abstract = "Purpose: We intended to determine whether transplanted neural precursors, derived from mouse embryonic stem (ES) cells, can migrate and differentiate into mature neurons and glial cells in damaged brains and improve functional deficits caused by global cerebral ischemic injury in adolescent rats. Methods: Global ischemia was induced using the four-vessel occlusion method. ES cells that display enhanced expression of yellow fluorescent protein were co-cultured in N2 supplemented media with PA6 cells that had stromal derived inducing activity. Neural precursor cells were directly transplanted bilaterally into hippocampal C3 areas 2. weeks after induction of global ischemia. Assessments of the Morris water-maze test at eight weeks and, the Open field activity levels at two, four, six and eight weeks after transplantation were carried out according to standard methods. Results: From neural precursors, we were able to generate neural lineages, including neurons and glial cells in vitro. Eight weeks following transplantation, cellular migration as well as generation of neural cells including neurons, astrocytes, and oligodendrocytes developed from the grafted ES cell-derived neural precursors were observed. Cell-transplanted animals exhibited enhanced functional recovery on sensorimotor and behavioral tests, compared to vehicle-treated control animals. Conclusion: Therefore, transplantation of mouse ES cell-derived neural precursor cells shows promise for improving recovery after global ischemia in adolescent rats.",
keywords = "Cell transplantation, Embryonic stem cells, Globally ischemic brain, Neural precursors",
author = "Kang, {Hoon Chul} and Dae-Sung Kim and Kim, {Ji Young} and Kim, {Han Soo} and Lim, {Bo Young} and Kim, {Heung Dong} and Lee, {Jin Sung} and Baik-Lin Eun and Kim, {Dong Wook}",
year = "2010",
month = "9",
day = "1",
doi = "10.1016/j.braindev.2009.09.010",
language = "English",
volume = "32",
pages = "658--668",
journal = "Brain and Development",
issn = "0387-7604",
publisher = "Elsevier",
number = "8",

}

TY - JOUR

T1 - Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats

AU - Kang, Hoon Chul

AU - Kim, Dae-Sung

AU - Kim, Ji Young

AU - Kim, Han Soo

AU - Lim, Bo Young

AU - Kim, Heung Dong

AU - Lee, Jin Sung

AU - Eun, Baik-Lin

AU - Kim, Dong Wook

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Purpose: We intended to determine whether transplanted neural precursors, derived from mouse embryonic stem (ES) cells, can migrate and differentiate into mature neurons and glial cells in damaged brains and improve functional deficits caused by global cerebral ischemic injury in adolescent rats. Methods: Global ischemia was induced using the four-vessel occlusion method. ES cells that display enhanced expression of yellow fluorescent protein were co-cultured in N2 supplemented media with PA6 cells that had stromal derived inducing activity. Neural precursor cells were directly transplanted bilaterally into hippocampal C3 areas 2. weeks after induction of global ischemia. Assessments of the Morris water-maze test at eight weeks and, the Open field activity levels at two, four, six and eight weeks after transplantation were carried out according to standard methods. Results: From neural precursors, we were able to generate neural lineages, including neurons and glial cells in vitro. Eight weeks following transplantation, cellular migration as well as generation of neural cells including neurons, astrocytes, and oligodendrocytes developed from the grafted ES cell-derived neural precursors were observed. Cell-transplanted animals exhibited enhanced functional recovery on sensorimotor and behavioral tests, compared to vehicle-treated control animals. Conclusion: Therefore, transplantation of mouse ES cell-derived neural precursor cells shows promise for improving recovery after global ischemia in adolescent rats.

AB - Purpose: We intended to determine whether transplanted neural precursors, derived from mouse embryonic stem (ES) cells, can migrate and differentiate into mature neurons and glial cells in damaged brains and improve functional deficits caused by global cerebral ischemic injury in adolescent rats. Methods: Global ischemia was induced using the four-vessel occlusion method. ES cells that display enhanced expression of yellow fluorescent protein were co-cultured in N2 supplemented media with PA6 cells that had stromal derived inducing activity. Neural precursor cells were directly transplanted bilaterally into hippocampal C3 areas 2. weeks after induction of global ischemia. Assessments of the Morris water-maze test at eight weeks and, the Open field activity levels at two, four, six and eight weeks after transplantation were carried out according to standard methods. Results: From neural precursors, we were able to generate neural lineages, including neurons and glial cells in vitro. Eight weeks following transplantation, cellular migration as well as generation of neural cells including neurons, astrocytes, and oligodendrocytes developed from the grafted ES cell-derived neural precursors were observed. Cell-transplanted animals exhibited enhanced functional recovery on sensorimotor and behavioral tests, compared to vehicle-treated control animals. Conclusion: Therefore, transplantation of mouse ES cell-derived neural precursor cells shows promise for improving recovery after global ischemia in adolescent rats.

KW - Cell transplantation

KW - Embryonic stem cells

KW - Globally ischemic brain

KW - Neural precursors

UR - http://www.scopus.com/inward/record.url?scp=77955424796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955424796&partnerID=8YFLogxK

U2 - 10.1016/j.braindev.2009.09.010

DO - 10.1016/j.braindev.2009.09.010

M3 - Article

C2 - 19854013

AN - SCOPUS:77955424796

VL - 32

SP - 658

EP - 668

JO - Brain and Development

JF - Brain and Development

SN - 0387-7604

IS - 8

ER -