Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method

Chang Mo Hwang, Woo Young Sim, Seung Hwan Lee, Amir M. Foudeh, Hojae Bae, Sang Hoon Lee, Ali Khademhosseini

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Poly(dimethylsiloxane) (PDMS) microstructures have been widely used in bio-microelectromechanical systems (bio-MEMS) for various types of analytical, diagnostic and therapeutic applications. However, PDMS-based soft lithographic techniques still use conventional microfabrication processes to generate a master mold, which requires access to clean room facilities and costly equipment. With the increasing use of these systems in various fields, the development of benchtop systems for fabricating microdevices is emerging as an important challenge in their widespread use. Here we demonstrate a simple, low-cost and rapid method to fabricate PDMS microstructures by using micropatterned poly(ethylene glycol) diacrylate (PEGDA) master molds. In this method, PEGDA microstructures were patterned on a glass substrate by photolithography under ambient conditions and by using simple tools. The resulting PEGDA structures were subsequently used to generate PDMS microstructures by standard molding in a reproducible and repeatable manner. The thickness of the PEGDA microstructures was controllable from 15 to 300 μm by using commonly available spacer materials. We also demonstrate the use of this method to fabricate microfluidic channels capable of generating concentration gradients. In addition, we fabricated PEGDA microstructures by photolithography from the light generated from commonly available laminar cell culture hood. These data suggest that this approach could be beneficial for fabricating low-cost PDMS-based microdevices in resource limited settings.

Original languageEnglish
Article number045001
JournalBiofabrication
Volume2
Issue number4
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Polydimethylsiloxane
Polyethylene glycols
Fabrication
Microstructure
Photolithography
Microtechnology
Costs and Cost Analysis
Controlled Environment
Microfluidics
Clean rooms
Microfabrication
Molds
Glass
Cell culture
Molding
Cell Culture Techniques
MEMS
baysilon
poly(ethylene glycol)diacrylate
Costs

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Hwang, C. M., Sim, W. Y., Lee, S. H., Foudeh, A. M., Bae, H., Lee, S. H., & Khademhosseini, A. (2010). Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method. Biofabrication, 2(4), [045001]. https://doi.org/10.1088/1758-5082/2/4/045001

Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method. / Hwang, Chang Mo; Sim, Woo Young; Lee, Seung Hwan; Foudeh, Amir M.; Bae, Hojae; Lee, Sang Hoon; Khademhosseini, Ali.

In: Biofabrication, Vol. 2, No. 4, 045001, 01.12.2010.

Research output: Contribution to journalArticle

Hwang, Chang Mo ; Sim, Woo Young ; Lee, Seung Hwan ; Foudeh, Amir M. ; Bae, Hojae ; Lee, Sang Hoon ; Khademhosseini, Ali. / Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method. In: Biofabrication. 2010 ; Vol. 2, No. 4.
@article{9e86d2cfea8b4d8b8938f60744bdfdfc,
title = "Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method",
abstract = "Poly(dimethylsiloxane) (PDMS) microstructures have been widely used in bio-microelectromechanical systems (bio-MEMS) for various types of analytical, diagnostic and therapeutic applications. However, PDMS-based soft lithographic techniques still use conventional microfabrication processes to generate a master mold, which requires access to clean room facilities and costly equipment. With the increasing use of these systems in various fields, the development of benchtop systems for fabricating microdevices is emerging as an important challenge in their widespread use. Here we demonstrate a simple, low-cost and rapid method to fabricate PDMS microstructures by using micropatterned poly(ethylene glycol) diacrylate (PEGDA) master molds. In this method, PEGDA microstructures were patterned on a glass substrate by photolithography under ambient conditions and by using simple tools. The resulting PEGDA structures were subsequently used to generate PDMS microstructures by standard molding in a reproducible and repeatable manner. The thickness of the PEGDA microstructures was controllable from 15 to 300 μm by using commonly available spacer materials. We also demonstrate the use of this method to fabricate microfluidic channels capable of generating concentration gradients. In addition, we fabricated PEGDA microstructures by photolithography from the light generated from commonly available laminar cell culture hood. These data suggest that this approach could be beneficial for fabricating low-cost PDMS-based microdevices in resource limited settings.",
author = "Hwang, {Chang Mo} and Sim, {Woo Young} and Lee, {Seung Hwan} and Foudeh, {Amir M.} and Hojae Bae and Lee, {Sang Hoon} and Ali Khademhosseini",
year = "2010",
month = "12",
day = "1",
doi = "10.1088/1758-5082/2/4/045001",
language = "English",
volume = "2",
journal = "Biofabrication",
issn = "1758-5082",
publisher = "IOP Publishing Ltd.",
number = "4",

}

TY - JOUR

T1 - Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method

AU - Hwang, Chang Mo

AU - Sim, Woo Young

AU - Lee, Seung Hwan

AU - Foudeh, Amir M.

AU - Bae, Hojae

AU - Lee, Sang Hoon

AU - Khademhosseini, Ali

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Poly(dimethylsiloxane) (PDMS) microstructures have been widely used in bio-microelectromechanical systems (bio-MEMS) for various types of analytical, diagnostic and therapeutic applications. However, PDMS-based soft lithographic techniques still use conventional microfabrication processes to generate a master mold, which requires access to clean room facilities and costly equipment. With the increasing use of these systems in various fields, the development of benchtop systems for fabricating microdevices is emerging as an important challenge in their widespread use. Here we demonstrate a simple, low-cost and rapid method to fabricate PDMS microstructures by using micropatterned poly(ethylene glycol) diacrylate (PEGDA) master molds. In this method, PEGDA microstructures were patterned on a glass substrate by photolithography under ambient conditions and by using simple tools. The resulting PEGDA structures were subsequently used to generate PDMS microstructures by standard molding in a reproducible and repeatable manner. The thickness of the PEGDA microstructures was controllable from 15 to 300 μm by using commonly available spacer materials. We also demonstrate the use of this method to fabricate microfluidic channels capable of generating concentration gradients. In addition, we fabricated PEGDA microstructures by photolithography from the light generated from commonly available laminar cell culture hood. These data suggest that this approach could be beneficial for fabricating low-cost PDMS-based microdevices in resource limited settings.

AB - Poly(dimethylsiloxane) (PDMS) microstructures have been widely used in bio-microelectromechanical systems (bio-MEMS) for various types of analytical, diagnostic and therapeutic applications. However, PDMS-based soft lithographic techniques still use conventional microfabrication processes to generate a master mold, which requires access to clean room facilities and costly equipment. With the increasing use of these systems in various fields, the development of benchtop systems for fabricating microdevices is emerging as an important challenge in their widespread use. Here we demonstrate a simple, low-cost and rapid method to fabricate PDMS microstructures by using micropatterned poly(ethylene glycol) diacrylate (PEGDA) master molds. In this method, PEGDA microstructures were patterned on a glass substrate by photolithography under ambient conditions and by using simple tools. The resulting PEGDA structures were subsequently used to generate PDMS microstructures by standard molding in a reproducible and repeatable manner. The thickness of the PEGDA microstructures was controllable from 15 to 300 μm by using commonly available spacer materials. We also demonstrate the use of this method to fabricate microfluidic channels capable of generating concentration gradients. In addition, we fabricated PEGDA microstructures by photolithography from the light generated from commonly available laminar cell culture hood. These data suggest that this approach could be beneficial for fabricating low-cost PDMS-based microdevices in resource limited settings.

UR - http://www.scopus.com/inward/record.url?scp=79953015337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953015337&partnerID=8YFLogxK

U2 - 10.1088/1758-5082/2/4/045001

DO - 10.1088/1758-5082/2/4/045001

M3 - Article

C2 - 21076185

AN - SCOPUS:79953015337

VL - 2

JO - Biofabrication

JF - Biofabrication

SN - 1758-5082

IS - 4

M1 - 045001

ER -