Beneficial effect of the unsaponifiable matter from rice bran on oxidative stress in vitro compared with α-tocopherol

Jin Won Lee, Seog Won Lee, Min Kyung Kim, Chul Rhee, In Hwan Kim, Kwang Won Lee

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Unsaponifiable matter (UM) was prepared from rice bran using n-hexane extraction followed by removal of its fatty acid methyl ester with supercritical CO2 under heat-stable conditions. The UM was made up of 1% of vitamin E isomers, 28% of γ-oryzanol and 71% of uncharacterized compounds. The aim of this study was to determine the antioxidant activities of the UM, using α-tocopherol (α-T) as a positive control, by measuring the Fe3+-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical-scavenging property and lipid peroxidation in rat liver microsomes. In addition, the effects of the UM on the tert-butyl hydroperoxide (t-BOOH)-induced cytotoxicity in cultured rat hepatocytes were also investigated. In FRAP assay and DPPH free-radical- scavenging assay, the results were expressed g-1 α-T or g -1 UM. The amount of UM used in lipid peroxidation assay and cytotoxicity assay was the amount required to have equal amounts of total vitamin E isomers in the sample and the control α-T. The UM, as well as α-T, exhibited significant antioxidant activities in FRAP, radical-scavenging and membrane-lipid oxidation. The FRAP value for total vitamin E isomers in the UM (TVEIUM) was 9.1 times higher than that for α-T. In terms of their capacities to perform radical-scavenging and lipid peroxidation, both TVEIUM and α-T showed similar antioxidant activities. In experiments using cultured rat hepatocytes, the t-BOOH-induced lactate dehydrogenase release was significantly inhibited by the addition of 63.5 and 160 μg ml-1 of TVEIUM treatments (84 and 89%, respectively), and that of 63.5 and 160 μg ml-1 of α-T treatment (88 and 93%, respectively). The antioxidant function against oxidative stress of the UM prepared from rice bran may extend its use to being a potential dietary supplement.

Original languageEnglish
Pages (from-to)493-498
Number of pages6
JournalJournal of the Science of Food and Agriculture
Issue number3
Publication statusPublished - 2005 Feb


  • Oxidative stress
  • Rice bran
  • Supercritical CO
  • Tocopherol
  • Unsaponifiable matter
  • γ-oryzanol

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics


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