Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers

Jae Min Lee, Ji Youn Lim, Yoonjin Kim, Ye Ji Kim, Hyuk Soon Choi, Eun Sun Kim, Bora Keum, Yeon Seok Seo, Yoon Tae Jeen, Hong Sik Lee, Soon Ho Um, Chang Duck Kim, Ho Sang Ryu, Donggeun Sul, Junghwa Hong, Hoon Jai Chun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study investigated benexate hydrochloride betadex (BHB)-mediated ulcer healing, and changes to microcirculation modulated through nitric oxide synthase (NOS) and anti-inflammatory activity. A rat model of gastric mucosal injury was established through injection of a 60% acetic acid solution into the stomach. Following ulcer induction, the rats were administered BHB orally for 5 days at doses of 0, 100, 300 or 1,000 mg/kg. The highest dose of BHB was also administered with or without L-NG-nitroarginine methyl ester (L-NAME). The area of gastric ulcers was determined by planimetry, and expression of cyclooxygenases (COX), cytokines and NOS in stomach tissues were measured using western blotting. Compared with the control group, gastric ulcer size was significantly decreased in the 1,000 mg/kg BHB-treated group (P<0.05). Administration of BHB led to a significant increase in endothelial (e)NOS expression (P<0.05). Although acetic acid co-treatment with L-NAME induced more severe mucosal damage, BHB decreased COX expression and tumor necrosis factor-α levels when administered with the nitric oxide inhibitor, L-NAME (P<0.05). BHB exhibited protective effects in a rat model of gastric ulcers, which were associated with a decrease in pro-inflammatory cytokine levels and the activation of eNOS.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalExperimental and Therapeutic Medicine
Volume12
Issue number2
DOIs
Publication statusPublished - 2016 Aug

Keywords

  • Benexate hydrochloride betadex
  • Cytokine
  • Nitric oxide
  • Stomach
  • Ulcer

ASJC Scopus subject areas

  • Immunology and Microbiology (miscellaneous)
  • Cancer Research

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  • Cite this

    Lee, J. M., Lim, J. Y., Kim, Y., Kim, Y. J., Choi, H. S., Kim, E. S., Keum, B., Seo, Y. S., Jeen, Y. T., Lee, H. S., Um, S. H., Kim, C. D., Ryu, H. S., Sul, D., Hong, J., & Chun, H. J. (2016). Benexate hydrochloride betadex modulates nitric oxide synthesis and cytokine expression in gastric ulcers. Experimental and Therapeutic Medicine, 12(2), 573-580. https://doi.org/10.3892/etm.2016.3384