Beta ig-h3 promotes renal proximal tubular epithelial cell adhesion, migration and proliferation through the interaction with α3β1 integrin

Sun Woo Park, Jong Sup Bae, Ki San Kim, Sun Hee Park, Byung Heon Lee, Je Yong Choi, Jae Yong Park, Sung Woo Ha, Yong Lim Kim, Tae Hwan Kwon, In San Kim, Rang Woon Park

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73 Citations (Scopus)

Abstract

Betaig-h3 (βig-h3) is a secretory protein composed of fasciclin I-like repeats containing sequences that allows binding of integrins and glycosaminoglycans in vivo. Expression of βig-h3 is responsive to TGF-β and the protein is found to be associated with extracellular matrix (ECM) molecules, implicating βig-h3 as an ECM adhesive protein of developmental processes. We previously observed predominant expression of βig-h3 expression in the basement membrane of proximal tubules of kidney. In this study, the physiological relevance of such localized expression of βig-h3 was examined in the renal proximal tubular epithelial cells (RPTEC). RPTEC constitutively expressed βig-h3 and the expression was dramatically induced by exogenous TGF-β1 treatment. βig-h3 and its second and fourth FAS1 domain were able to mediate RPTEC adhesion, spreading and migration. Two known α3β1 integrin-interaction motifs including aspartatic acid and isoleucine residues, NKDIL and EPDIM in βig-h3 were responsible to medicate RPTEC adhesion, spreading, and migration. By using specific antibodies against integrins, we confirmed that α3β1 integrin mediates the adhesion and migration of RPTECs on βig-h3. In addition, it also enhanced proliferation of RPTECs through NKDIL and EPDIM. These results indicate that βig-h3 mediates adhesion, spreading, migration and proliferation of RPTECs through the interaction with α3β1 integrin and is intimately involved in the maintenance and the regeneration of renal proximal tubular epithelium.

Original languageEnglish
Pages (from-to)211-219
Number of pages9
JournalExperimental and Molecular Medicine
Volume36
Issue number3
Publication statusPublished - 2004 Jun 30

Keywords

  • Adhesion
  • Migration
  • Proliferation
  • Renal proximal tubular epithelial cell
  • α3β1 integrin
  • βig-h3

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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  • Cite this

    Park, S. W., Bae, J. S., Kim, K. S., Park, S. H., Lee, B. H., Choi, J. Y., Park, J. Y., Ha, S. W., Kim, Y. L., Kwon, T. H., Kim, I. S., & Park, R. W. (2004). Beta ig-h3 promotes renal proximal tubular epithelial cell adhesion, migration and proliferation through the interaction with α3β1 integrin. Experimental and Molecular Medicine, 36(3), 211-219.