Bidirectional Alterations in Cerebellar Synaptic Transmission of tottering and rolling Ca2+ Channel Mutant Mice

Kaori Matsushita, Minoru Wakamori, Im Joo Rhyu, Tatsuo Arii, Sen Ichi Oda, Yasuo Mori, Keiji Imoto

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Abstract

Hereditary ataxic mice, tottering (tg) and rolling Nagoya (tg rol), carry mutations in the P/Q-type Ca2+ channel α1A subunit gene. The positions of the mutations and the neurological phenotypes are known, but the mechanisms of how the mutations cause the symptoms and how the different mutations lead to various onset and severity have remained unsolved. Here we compared fundamental properties of excitatory synaptic transmission in the cerebellum and roles of Ca2+ channel subtypes therein among wild-type control, tg, and tgrol mice. The amplitude of EPSC of the parallel fiber-Purkinje cell (PF-PC) synapses was considerably reduced in ataxic tgrol. Although the amplitude of the parallel fiber-mediated EPSC was only mildly decreased in young non-ataxic tg mice, it was drastically diminished in adult ataxic tg mice of postnatal day 28-35, showing a good correlation between the impairment of the PF-PC synaptic transmission and manifestation of ataxia. In contrast, the EPSC amplitude of the climbing fiber-Purkinje cell (CF-PC) synapses was preserved in tg, and it was even increased in tgrol, which was associated with altered properties of the postsynaptic glutamate receptors. The climbing fiber-mediated EPSC was more dependent on other Ca2+ channel subtypes in mutant mice, suggesting that such compensatory mechanisms contribute to maintaining the CF-PC synaptic transmission virtually intact. The results indicate that different mutations of the P/Q-type Ca2+ channel not only cause the primary effect of different severity but also lead to diverse additional secondary effects, resulting in disruption of well balanced neural networks.

Original languageEnglish
Pages (from-to)4388-4398
Number of pages11
JournalJournal of Neuroscience
Volume22
Issue number11
Publication statusPublished - 2002 Jun 1

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Keywords

  • Calcium channel
  • Cerebellar ataxia
  • Glutamate receptor
  • Mutant mice
  • Rolling Nagoya mice
  • Synaptic transmission
  • Tottering mice

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Matsushita, K., Wakamori, M., Rhyu, I. J., Arii, T., Oda, S. I., Mori, Y., & Imoto, K. (2002). Bidirectional Alterations in Cerebellar Synaptic Transmission of tottering and rolling Ca2+ Channel Mutant Mice. Journal of Neuroscience, 22(11), 4388-4398.