Abstract
In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.
| Original language | English |
|---|---|
| Pages (from-to) | 5661-5665 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 580 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 2006 Oct 16 |
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Keywords
- Bone resorption
- Glutathione
- Osteoclast differentiation
- Reactive oxygen species
- Redox status
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts. / Kim, Hyunsoo; Kim, Ick Young; Lee, Soo Young; Jeong, Daewon.
In: FEBS Letters, Vol. 580, No. 24, 16.10.2006, p. 5661-5665.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bimodal actions of reactive oxygen species in the differentiation and bone-resorbing functions of osteoclasts
AU - Kim, Hyunsoo
AU - Kim, Ick Young
AU - Lee, Soo Young
AU - Jeong, Daewon
PY - 2006/10/16
Y1 - 2006/10/16
N2 - In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.
AB - In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.
KW - Bone resorption
KW - Glutathione
KW - Osteoclast differentiation
KW - Reactive oxygen species
KW - Redox status
UR - http://www.scopus.com/inward/record.url?scp=33749534719&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749534719&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2006.09.015
DO - 10.1016/j.febslet.2006.09.015
M3 - Article
C2 - 16996506
AN - SCOPUS:33749534719
VL - 580
SP - 5661
EP - 5665
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 24
ER -