Abstract
In order to demonstrate that cellular redox status undergoes decreased reduction during osteoclast differentiation and further decreased reduction during osteoclastic bone resorption, we analyzed γ-glutamylcysteinyl synthetase activity, a glutathione synthesis rate-limiting enzyme, and total glutathione and thiol groups. Moderate and severe redox shifts towards a more oxidizing environment induced gradual increases and decreases in osteoclastogenesis. Moreover, while severe glutathione depletion inhibited bone resorption, moderate glutathione repletion enhanced bone resorption. In summary, our observations suggest that there is a threshold for redox status, representing biphasic patterns in osteoclast differentiation and function.
Original language | English |
---|---|
Pages (from-to) | 5661-5665 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 580 |
Issue number | 24 |
DOIs | |
Publication status | Published - 2006 Oct 16 |
Keywords
- Bone resorption
- Glutathione
- Osteoclast differentiation
- Reactive oxygen species
- Redox status
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology