Binding characteristics of a bacterial expansin (BsEXLX1) for various types of pretreated lignocellulose

In Jung Kim, Hyeok Jin Ko, Tae Wan Kim, Ki Hyun Nam, In Geol Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

BsEXLX1 from Bacillus subtilis is the first discovered bacterial expansin as a structural homolog of a plant expansin, and it exhibited synergism with cellulase on the cellulose hydrolysis in a previous study. In this study, binding characteristics of BsEXLX1 were investigated using pretreated and untreated Miscanthus x giganteus in comparison with those of CtCBD3, a cellulose-binding domain from Clostridium thermocellum. The amounts of BsEXLX1 bound to cellulose-rich substrates were significantly lower than those of CtCBD3. However, the amounts of BsEXLX1 bound to lignin-rich substrates were much higher than those of CtCBD3. A binding competition assay between BsEXLX1 and CtCBD3 revealed that binding of BsEXLX1 to alkali lignin was not affected by the presence of CtCBD3. This preferential binding of BsEXLX1 to lignin could be related to root colonization in plants by bacteria, and the bacterial expansin could be used as a lignin blocker in the enzymatic hydrolysis of lignocellulose.

Original languageEnglish
Pages (from-to)5381-5388
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume97
Issue number12
DOIs
Publication statusPublished - 2013 Jun

Keywords

  • Bacterial expansin
  • Binding
  • BsEXLX1
  • Cellulose
  • Lignin
  • Lignocellulose
  • Miscanthus x giganteus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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